Expression And Clinical Significance Of HIF-1α And MIF In Prostate Carcinoma

Background and Objective:Prostate carcinoma is the most common urinary tract tumor.and along with the age growth, its incidence has obvious regional differences, the United States and Europe is higher.The American cancer society estimates that in2014new cases of prostate cancer in the United States is about233000cases, accounting for27%of all cancer cases, death cases (29480cases), second only to lung cancer.In our country, its incidence shows a rising trend year by year.The pathogenesis of PC has not yet been clarified, and the level of regulation by multiple genes may be involved in many important aspects of the disease. This article was to investigate the expression of HIF-la and MIF in prostate cancer tissue, to explore the relationship between both and prostate cancer’s Gleason score, clinical stage, age. To study whether there is correlation between expression of HIF-la and MIF in prostate cancerMethods:We collected52cases of prostate cancer which were all confirmed by pathology specimens from prostate biopsy or surgery and from second hospital of Tianjin medical university in February2009-February2012.And twenty cases diagnosed benign prostatic hyperplasia in the same period were randomly chosen as comparison.Hematoxylin and eosin stained slides period were preformed in all cases and then re-confirmed the diagnosis by pathology.The expression of HIF-la and MIF in52prostate cancer and20benign prostatic hyperplasia were assessed by immunohistochemistry. The result of immunohistochemical staining were observed by light microscope, and then make analysis. SPSS19.0software was employed for statistical analysis. Chi-square test or Fisher"s exact test was used to analyze the association of expression of HIF-la and MIF in BPH and prostate cancer. The correlation between expression of HIF-la and MIF and clinicopathological features (age, PSA, Gleason score, TNM staging) of patients with prostate cancer was analyzed with a Chi-square test or Fisher’s exact test. Spearman rank correlation analysis was used to analyze the correlation of HIF-la and MIF expression in prostate cancer.All analysis and statistical significance was defined as P values less than0.05. Results:(1)In prostatic tissue, HIF-lα expressed mainly in nuclei, and MIF localized in the cytoplasm. HIF-la positive stained rate was significant higher in PC (67.31%) than that in BPH (15.00%). MIF positive stained rate was significant higher in PC (78.85%) than that in BPH (40.00%). There were significant differences between PC group and BPH group (P<0.01).(2)The expression of HIF-la and MIF was significantly associated with T stage, N stage and distant metastasis. Further, MIF expression showed a correlation with Gleason score (P<0.05).Both of HIF-la and MIF expression was not significantly related to the age and PSA level.(3)In33cases of PC, the staining of HIF-la and MIF is positive commonly. Using Spearman Rank Correlation Test, there was a positive correlation between the expressions of HIF-la and MIF in prostatic carcinoma (r=0.542, P<0.01).Conclusions:1.HIF-la and MIF were up-regulated in prostatic carcinoma.There was significant difference between the PC and BPH.The result suggests that HIF-la and MIF play an important role in formation of prostate cancer.2.The expression of HIF-1α、MIF was significantly associated with organ-confined, lymph node metastasis, distant metastasis.The expression of MIF increased with the increase of the Gleason score levels.The result suggests that the interaction of HIF-la and MIF promote the development,invasion and metastasis of prostate cancer.3.The expression of HIF-la was positive correlation with MIF protein expression in prostate cancer.Combined detection HIF-la and MIF have some referred value to the initiation、proliferation and prognosis of prostate carcinoma.