The Effects Of Ammonium Pyrrolidinedithiocarbamate And Cisplatin On Proliferation And Apoptosis Of Non-small-cell Pulmonary Carcinoma A549Cells In Vitro

Objective: To observe the effect of Pyrrolidinedithiocarbamate and cisplatin onthe proliferation and apoptosis of human non-small cell lung cancer in vitro，and therelationship between the expression of Bcl-2and Bax and NF-kBp65．Methods: Different drug concentrations PDTC, DDP and DDP combined effectof PDTC after48h, under an inverted microscope to directly observe changes in cellmorphology after administration of each group, after HE staining in each group afterthe administration change cell morphology under light microscope; MTT method testPDTC, DDP and DDP and PDTC cytotoxic non-small cell lung cancer A549cells andobserve whether there is a dose and time dependent; immunohistochemical stainingwas observed PDTC, DDP and DDP and PDTC apoptosis in non-small cell lungcancer A549cells. protein Bcl-2, Bax and NF-kBp56expression changes of.Results:1. Effects of PDTC,DDP,PDTC and DDP on the A549cell apoptosis:different drug concentrations (PDTC, DDP,PDTC+DDP) role cells after48hoursunder an inverted microscope to see: A549cells with increasing drug concentration,cell density decreased ability becomes adherent poor, some cells round and shrunkenand float in the culture medium; after HE staining under light microscopy: A549cellswith increasing concentrations of the drug, the cell density decreased retention,changes in cell morphology, nuclear proportion of pulp decreases, the cells appearedshrinkage, nuclear membrane lysis, chromatin condensation, culminating in apoptosis.2. Effects of PDTC, DDP, DDP and PDTC on A549cell proliferation:(1).Different drug concentrations PDTC, DDP and PDTC+DDP inhibited theproliferation of A549cells, and with time and concentration, inhibition rates were increased to36.18%from3.12%, from3.54%to39.91%, and an increase from12.19%to63.3%, there is a time and concentration-dependent; and each drug groupwere compared with the control group, the difference in growth inhibition of A549cells were statistically significant (P <0.05).(2). After48hours, the drug group PDTC,DDP and PDTC+DDP on A549cell proliferation inhibition rates were8.89%,16.41%,19.58%,30.64%,5.3%,14.74%,24.39%,39.51and13.33percent,25.13%,39.85%,58.5%, the first two groups were statistically significant differences betweenthe DDP and PDTC group (P <0.05), Q values greater than1.15, which means thatthe combined effects of PDTC+DDP in A549cells have a synergistic effect.3. PDTC,DDP,PDTC and DDP A549cells expressing the protein byimmunohistochemistry: PDTC,DDP,PDTC and DDP different concentrations withincreasing concentrations of protein NF-kBp65reduced by Bal-2expression ofanti-apoptotic proteins, pro-apoptotic proteins Bax expression increased.Conclusion:1.PDTC and DDP can inhibit the proliferation of esophageal cancerA549cells and its make a dose-time-dependent manner;2. PDTC and DDP in asynergistic effect in A549cells, inhibit cell proliferation and promote apoptosis;3.Further confirmed, PDTC inhibited by NF-κBp65protein, inhibition of Bcl-2proteinexpression of anti-apoptotic protein, thereby promoting apoptosis, PDTC can promoteDDP chemotherapy.