Detection Of P53, And P16Protein Expression And Deletion Of Chromosome5Q In MDS

Objective1. To observe the expressions of P53, and P16protein in MDS (myelodysplastic syndrome) and explore the value in diagnosis, typing of MDS;2. To observe the delect ion of chromosome5q in MDS and explore the value in diagnosis, typing of MDS.Methods1. To collect46cases of MDS of the First Affiliated Hospital of Guangzhou University of TCM which were confirmed by clinical, peripheral blood, bone marrow biopsy and bone marrow smear, cytogenetics detection in2005-2013, review the clinical history, archive smear and biopsy section, and the data of treatment and prognosis again.2. Bone marrow biopsy tissue embedded in wax of MDS were sliced up again:(1) Using immunohistochemistry to detect the expression of MPO, CD61, CD71,P53, and P16, etc;(2) Using fluorescence in situ hybridization technique to detect the deletion of chromosome5q.3. Controls:5cases of normal bone marrow and5cases of giant cell anemia were used as controls, the negative and positive control were set each batch experiment at the same time.ResultsHigh expression of P53, and P16were detected in MDS, the expression rate was76%and28.3%respectively. No expression was detected in normal bone marrow and gigantic young cell anemia; The expressions of P53and P16have significant trend, but the expression rate of P53, and P16in MDS subtypes have no statistical difference (2) The deletion of5q was detected in13.04%of MDS.Conclusion(1) High expression of P53, and P16were detected in MDS, the expression rate was76%and28.3%respectively. The expressions of P53, P16in MDS has reference value in the distinguish between benign hyperplastic anemia and MDS, but have no reference value in subtyping of MDS;The expressions of P53and P16have significant trend, P53may acts through cell cycle regulation related to P16(2) The deletion of5q existed in13.04%of MDS, the deletion of5q in MDS has reference value in the distinguish between benign hyperplastic anemia and MDS.