Research On Gene Level Of Small Intestine Secreting Insulin In Rats

Objective:To clear the existence and the expression level of small intestine insulin mRNA and use of small animal imaging techniques to prove the small intestine to secrete insulin.Methods:The Wister rats of eight week78by irrigation with bovine serum albumin solution, Oil suspension and50%glucose solution were executed at0min,5min,10min,15min,20min,25min, Different time period was divided into six groups, then got its duodenum, pancreas, extracting the tissue total RNA, Reverse transcriptase PCR gel electrophoresis detected the existence of insulin mRNA of every tissue real-time fluorescence PCR detected insulin mRNA expression of every part of pancreatic and duodenum. Using small animal imaging Cy7NHS-labeled anti-rat insulin antibody The mice were placed in Kodak in Vivo Imaging System for fluorescence imaging, an excitation wavelength720nm, emission wavelength790nm, using software for analysis.Results:Common PCR amplificated gene sequencing in the small intestine insulin of rats. Using real-time fluorescent quantitative PCR, Detecting the insulin mRNA relative expression of pancreas was significantly higher than the amount of duodenum (P<0.05).The insulin expression level of pancreas and duodenum were dynamic changing, and the insulin expression peak of the pancreas was higher than. Duodenum Small animal imaging observed that fluorescence signal is higher than the small intestine tissues except the pancreasConclusion:1. This trial confirms that the small intestine tissue secrete insulin And after gavaging to rats relative expression of duodenal insulin mRNA showed a dynamic table changing.2. Real-time monitoring of in vivo imaging techniques monitor insulin secretion in rats. We speculate diabetes another possibility pathogenesis.