The Mechanism Of SENP1Affecting Drug Resistance In Hepatocellular Carcinoma

Objective:In order to explore the role of SENP1in drug resistance of hepatocellular carcinoma, we test the influence of SENP1to drug sensitivity, and the relative expression of SENP1mRNA and XBP1mRNA in Clinical specimens, so that to provide new theoretical foundation for comprehensive treatment and the target of drug treatment targets of hepatocellular carcinoma.Methods:1. We transfect SENP1and its mutant type SENP1m (R630L,K631M) to HCC cell line HepG2, use Western Blot technique to detect the expressions of SENP1and SENP1m, and use flow cytometry to test the cell apoptosis rates of HCC cell line HepG2transfected by SENP1and SENP1m under different concentrations of5-Fu.2. We use RT-PCR technique to test the relative expression of SENP1mRNA and XBP1mRNA in HCC tissues and corresponding paracarcinoma tissues.Then we studied the relationship between the relative expression of SENP1mRNA and XBP1mRNA.Results:1. The apoptosis rate of HepG2transfected by SENP1was lower than that of HepG2transfected by SENP1m.2. Compared with corresponding paracarcinoma tissues, the relatively expression level of SENP1mRNA and XBP1mRNA in HCC tissues was higher, while the expression of XBP1mRNA was positively correlated with SENP1mRNA (p<0.001)Conclusion:Our research showed that the apoptosis rate of HepG2transfected by SENP1was lower than that of HepG2transfected by SENP1m, and compared with corresponding paracarcinoma tissues, the relatively expression level of SENP1mRNA and XBP1mRNA in HCC tissues was higher, while the expression of XBP1mRNA was positively correlated with SENP1mRNA. These suggest that SENP1influences the drug sensitivity to5-Fu of HepG2cells, and may inhibit apoptosis or promote survival by adjusting the unfolded protein response when the endoplasmic reticulum is in a state of stress, thus to make drug resistance of hepatocellular carcinoma.