Test And Significance Of Treg/Th17Cells In The Serum Of AECOPD Patients With Different Airway Responsiveness

Objective:(1)To explore the change and significance of CD4+CD25+CD127low Regulatory T cell (Treg cell)and Th17cell-specific cytokines:interleukin17(IL17) in acute stage COPD and stable COPD.(2)To explore the significance and the difference of level of CD4+CD25+CD127low Regulatory T cell (Treg cell)and Th17cell in peripheral boood of patients of acute stage COPD with different airway responsiveness.Methods:26inpatients with acute stage COPD checkd by pulmonary function test who the bronchial dilation test is negative,20inpatients with acute stage COPD checkd by pulmonary function test who the bronchial dilation test is positive,20follow-up visit patients with stable COPD,and15healthy person as control group. Flow cytometry was used to detect the proportion of Treg cell in peripheral boood,ELISA method was used to detect the expresstion of IL17in peripheral boood, the proportion of hemocyte and immune globulin in peripheral boood was determined meanwhile.Results:(1)The proportion of Treg cell in group AECOPD increased significantly compared with group stable COPD and control group(P<0.001). Group stable COPD is higher than control group,but there were no significance. The level of IL17in peripheral boood in group AECOPD and group stable COPD increased significantly compared with control group(P<0.001); Group AECOPD was higher than stable group COPD(P<0.01),Group COPD was higher than control group(P=0.046). There was not correlated between the proportion of Treg cell and the level of IL17and other indicators in either group.General clinical features were compared between group AECOPD, group COPD and the healthy control group. FEV1%and FEV1/FVC indicators which were reflected the level of lung function, group AECOPD and group COPD were lower than the control group, but no significant difference between the group COPD and group AECOPD; Immunoglobulin IgG, IgA, IgM which were responsed immunologic function were not statistically significant between the three groups; Comparison of proportions of blood cells:group AECOPD WBC absolute value higher than group COPD and the control group.there were statistical differences, and no significant difference between group COPD and the control groups; group AECOPD and group COPD neutrophil proportion N%were increased compared with the control group, there were statistical differences; group AECOPD and group COPD lymphocyte proportion L%lower than the control group, statistical differences.(2)The proportion of Treg cell in group AECOPD combined with bronchial dilation test was negative and group AECOPD combined with bronchial dilation test was positive increased significantly compared with control group (P<0.001). Group bronchial dilation test was negative higher than group bronchial dilation test was positive (P<0.001).The level of IL17in peripheral boood in group AECOPD combined with bronchial dilation test was negative and group AECOPD combined with bronchial dilation test was positive increased significantly compared with control group (P<0.001). Group bronchial dilation test was negative lower than group bronchial dilation test was positive (P=0.023).The proportion of Treg cell and the level of IL17in group AECOPD combined with bronchial dilation test was positive was significantly negative correlated. The proportion of Treg cell was negative correlated with FEV1%.Group clinical features were comparied between AECOPD negative group, AECOPD positive group and the healthy control. FEV1%and FEV1/FVC indicators which were reflected the level of lung function, AECOPD negative group and AECOPD positive group were lower than the control group, but there were no significant difference between the AECOPD negative group and AECOPD positive group; Immunoglobulin IgG, IgA, IgM which were responsed immunologic function were not statistically significant between the three groups; Compasion of proportions of blood cells:AECOPD negative group WBC absolute value higher than the control group.It was higher than AECOPD positive group also, but was no statistical different. There was no statistically significant difference between AECOPD positive group and the control group; AECOPD negative group and AECOPD positive group proportion of neutrophils N%increased compared with the control group, there were statistical differences, but there was no statistical difference between AECOPD positive group and the control group; AECOPD negative group and AECOPD positive group lymphocyte proportion L%lower than the control group, statistical differences, but there was no statistical difference between AECOPD positive group and the control group. AECOPD negative proportion eosinophils lower than the control group, but no significant difference, AECOPD positive group the proportion of eosinophils increased compared with the control group, but was no significant different. AECOPD positive peripheral blood eosinophils proportion (E%) higher than AECOPD negative group, there was statistical differences (P=0.031).(3)The proportion of Treg cell in group AECOPD combined with bronchial dilation test was positive higher than group stable COPD (P<0.05).The level of IL17in peripheral boood in group AECOPD combined with bronchial dilation test was positive higher than group bronchial dilation test was positive (P<0.05).Conclusions:(1)The abnormal of lever of Treg cell and IL17in AECOPD and COPD meant the autoimmune mechanism which Treg/Thl7cell may be involved in may exist in different stage of COPD. The break of balance of Treg cell and Thl7cell may induce the disorder of immune and continue inflammation.(2)Treg/Thl7cells play a role of immune-adjustment function in different airway responsiveness of AECOPD, but the immune-adjustment function may exit differrence. The research of Regulatory T cells and Th17cell may can provide new ideas in the research of COPD combined with airway hyperresponsiveness.