| BackgroundBecause of its good effects and no pain during the treatment, ECS has been applied in the field of paychiatric treatment for more than70years in the psychiatric clinical application. But the treatment mechanism of ECS is not yet clear,so it is very necessary to discuss the mechanism related to ECS, which would be the entrance to explore pathological mechanism of mental illness. In recent years, the application of ECS is beyond the field of paychiatric treatment. Therefore, it is very necessary to discuss the relevant mechanism of ECS.ObjectivesTo observe the influence of ECS on hippocampal synaptic electrophysiological characteristics and the structure in normal SD rat, the mechanism of ECS is explored, in order to provide some clues for further exploring the pathogenesis of mental illness.Mothods1. Selected60healthy adult and250-300g male SD rats, they were randomly divided into ECS group (50rats) and control group (10rats). Group ECS:animal to receive daily1ECS (ECS stimulation parameters:pulse frequency of100Hz, pulse duration of0.5ms, convulsive duration is1.0s, electric flow of60mA), for14consecutive days, a total of14times.The control group are expored to the same parameters compared with ECS groups, but without electricity.1times a daily,14times in a row.2. After14ECS times, ECS groups (totally30rats) were randomly selected4rats every group respectively in the electrophysiology in0,2,4,6,8,10, and12days (denoted with ECSD0, ECSD2, ECSD4, ECSD6, ECSD8, ECSD10and ECSD12group); The control group were randomly selected4rats with14shame stimulation. By using extracellular recording technique, we observed the discharge activity in each basic biological, background stimulus intensity and long term potentiation (long-term potentiation, LTP) changes in hippocampus CA1area of rats.3. After14ECS times, ECS groups (totally20rats) were randomly selected4rats every group in the electron microscope in0and7days (denoted with ECSDO, ECSD7); The control group were randomly selected4rats with14shame stimulation. By using electron microscope, we observed synapses and synaptic interface structure changes in hippocampus CA1area of rats.4. Statistical analysis was performed using SPSS software (version17.0, SPSS). Results are presented as mean±SD. Using independent samples t test was used for ECS groups and control group. All other measures were analyzed using one-way analysis of variance at different time point. The significance level was set to a=0.05.Results1. Effects of ECS on physiological activity of hippocampus neuronsSpontaneous discharge frequency of ECSDO, ECSD2, ECSD4, ECSD6, ECSD8, ECSD10and ECSD12increased compared with control group, the difference was statistically significant (P=0.000,0.000,0.000,0.029,0.000,0.000,0.029); The average amplitude of ECSD2, ECSD4, ECSD6, ECSD8, ECSD10, ECSD12increased compared with control group, the difference was statistically significant (P=0.029,0.000,0.025,0.000,0.000,0.029); Optimal stimulation amplitude of ECSDO, ECSD2, ECD4, ECSD6, ECSD8, ECSD10, ECSD12increased compared with control group, the difference was statistically significant (P=0.000,0.000,0.000,0.000,0.000,0.000,0.000).Inducing ratio, depolarization slope, and amplitude of LTP of ECSDO, ECSD2, ECSD4, ECSD6, ECSD8, ECSD10, ECSD12declined compared with control group, the difference was statistically significant (P>=0.000,0.000,0.000,0.000,0.000,0.000,0.000); Repolarization slope of ECSDO, ECSD2, ECSD4, ECSD6, ECSD8, ECSD10, ECSD12declined compared with control group, the difference was statistically significant (P=0.000,0.000,0.000,0.000,0.008,0.000,0.008); Population spike of ECSDO, ECSD2, ECSD4, ECSD10declined compared with control group, the difference was statistically significant (F=0.106,0.112,0.394,0.976,0.394,0.332,0.632).2. Effects of ECS on synaptic structural plasticity in ratsThe width of synaptic cleft of ECSDO, ECSD7declined compared with control group, the difference was statistically significant (P=0.000,0000); The thickness of the postsynaptic density of ECSDO, ECSD7declined compared with control group, the difference was statiscally significant (P=0.000,0.000); Length of synaptic active zone of ECSDO, ECSD7declined compared with control group, the difference was statiscally significant (P=0.000,0.000); The synaptic numerical density of ECSDO, ECSD7declined compared with control group, the difference was statiscally significant (P=0.003,0.000); The average area of synapse of ECSDO, ECSD7enlarged compared with control group, the difference was statiscally significant (P=0.000,0.002). Conclusions1. ECS influences neural electrophysiological activity of central nervous system, characterized by increasing spontaneous discharge activity in the early phase and inhibiting LTP induction in the following phase and decreasing the activity of LTP, suggesting that ECS influences synaptic splaticity through the inhibition of synaptic function transferring.2. ECS influences neural synaptic modifications of central nervous system, characterized by reducing the width of synaptic cleft, reducing the thickness of postsynaptic dense, shortening the synaptic activity zone length, reducing the number of synaptic density and increasing synaptic synaptic average area to influence the transmission of nerve function, and then to adjust the nerve function. |
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