A Quantitative Study Of Application Of MSCTA In Wound Blood Result Closed Damage In A Rabbit Model

ObjectiveThe purpose of this study was to inspect the closed vessel damage of fourlimbs resulted from trauma. The scans were performed with MSTCA,andcompared with the pathology and which provide the theoretical basis for theearly diagnosis and treatment of the closed vessel damage resulted from trauma.Materials and methods1.MaterialsThirty-two New Zealand White Rabbits were selected,weight2.1kg~3.4kg,on average2.6kg.Quantitative strikes were executed on the lower side of hindlimbs,and compared with the normal side.After the strikes,all limbs werereceived the conventional CT scans and CTA examination separately. Experimental animals were executed after the experiment in5~10minutes. Damagedvessels (arteriae iliaca externa) were taken out and conducted the HE dryingexaminations.2.Observation indexes2.1MSCTA inspections:Lower hind limbs of rabbits were damagedaccording to the predetermined plan,and were received the128spiral CTangiography examinations.Then the original images were sent to the image post-processing workstation.Image post-processing software was used to analyze thecharacteristics of the damaged vascular on the lower sides of the hind limbs,andthe running and filling of the MSCTA vessel.Cases with vessels stenosis weremeasured by the standards of peripheral vascular stenosis,and which can bedivided into5degrees according to the international standards. 2.2Making specimen of the damaged vascular:After MSCTA examinationsin5~10minutes,the experimental animal were executed instantly.The specimenwere cut through on the basis of3mm distances,and the each sections werenumbered,and were put into the10%formalin fluids,fixed, and embedded.TheH-E drying is used to make the morphology observation for the vascular,and theSchwarts method was used to calculate thickness and stenosis rate of vascularneointimal.3.Statistical analysisThe Kappa coefficient was used to check and compare the consistency onthe classification between MSCTA of the rabbit’s damaged vessel on the lowerhind limbs and pathology.Results1.MSCTA inspection of the32rabbits with the damaged vessel on thelower hind limbs:32rabbits with damaged limbs were selected to receive theMSCTA examination,6cases of normal vessel:26cases of damaged vessel:5cases of artery bruises and blocking;2cases of pseudoaneurysm;6cases ofobvious exosmose of contrast medium;9cases of blood vessel of localstenosis,4cases of blood vessel of beaded stenosis.2.Morphological changes of the32rabbits with the damaged vessel on thelower hind limbs:this group of obvious fracture is26cases,9cases of obviousdisplacement of fracture segments,14cases of mild displacement;3cases offracture segments with different degrees of hematocele but no displacement;6cases of obvious breaking blood vessel;18cases of different degrees bruise;2case of pseudoaneurysm.Conclusion1.The design of this experiment is simple and easy to be operated and easyto be repeated,and the characteristics of damaged vascular and pathologicalclassification are closer to the trauma of emergency.2. MSCTA can clearly display the location and degree of damaged vascularand show the relationships between the damaged vascular and the surroundingbone fracture,he classification of damaged vascular and the degrees of stenosisare consistent with the pathology.