Research On Free Fatty Acid Levels Of The Simple Obese Children

Objective: The simple obesity is turning into a common childhood disease at present. Itleads to the fat deposition and insulin resistance. Due to these situations, the morbidityof diabetes is increasing in children. Therefore, this study was to investigate theconcentrations of free fatty acid (FFA) in simple obese children. Then assess therelationship between insulin resistance (IR) and FFA. It could help to improve thesituation of modern children’s physical fitness.Methods: The study was conducted of forty-five children(7to14years old). Theyhospitslized the Pediatric inpatient department of the Second Affiliated Hospital ofDalian Medical University(from September2013to March2014). Their height andbody weight were measured and then calculated the BMI. According to the BMIclassification standard for Chinese school-age teenager developed by Chinese obesityworking group in2004,25obese children were selected to be the obesity group and20normal children were selected to be the control group. All of them did not have anyinfection and other non-infectious diseases. The concentrations of fasting glucose,triglyceride and total cholesterol in serum were determined by automatic biochemicalanalyzer. The concentrations of fasting insulin in serum were determined by radioimmunity method. The concentrations of FFA were determined by ELISA. Insulinsensitivity was evaluated by Homeostasis model assessment-insulin resistance(HOMA-IR) index. HOMA-IR=FPG×FINS/22.5.Results:1.The BMI in the obesity group was significantly higher than that in the controlgroup(28.77±3.63vs16.99±1.48kg/m2, P＜0.01).2.Compaired to the control group, the obesity group had the significantly higherconcentrations of fasting insulin (19.32±7.68vs8.56±2.26uU/ml, P＜0.01) and the HOMA-IR (4.11±1.68vs1.74±0.48, P＜0.01) respectively. In the obesity group, theconcentration of fasting glucose was (4.77±0.32)mmol/L. In the control group heconcentration of fasting glucose was (4.58±0.38)mmol/L. The difference between twogroups was not significant(P=0.082).3.The obesity group had the significantly higher concentration of FFA incomparison to the control group(9.18±3.08vs4.90±1.75ng/ml, P＜0.01). The obesitygroup had the significantly higher concentrations of triglyceride (1.52±0.61vs0.82±0.22mmol/L, P＜0.01) and total cholesterol (4.26±0.60vs3.75±0.47mmol/L, P＜0.01) in comparison to the control group respectively.4.In the obesity group, the concentration of fast insulin and insulin resistance weresignificant positive correlations with BMI respectively(r=0.795,0.798, P＜0.01).5.In the obesity group, the FFA level was significantly and positively related toBMI and HOMA-IR respectively(r=0.677,0.841, P＜0.01).Conclusion:1.The FFA level of simple obese children was significantly higher than those ofnormal children.2.The HOMA-IR was significantly higher in simple obese children compaired withnormal children, and it was positively correlated with BMI. It suggested that simpleobese children prone to having insulin resistance.3.There was the positive relationship between the FFA level and insulin resistancein simple obese children. It suggested that FFA involves in the mechanism of insulinresistance in simple obese children.