KPC-producing Klebsiella Pneumoniae Resistance Research

Objective:By collection and analysis of Klebsiella pneumoniae from the First Affiliated Hospital of Shanxi medical university of2013, to study the resistance characteristics and its resistance mechanisms.8patients and clinical data were retrospectively analyzed and summarized the characteristics of clinical cases.Through its treatment programs, results, discuss the treatment infection progress of Klebsiella pneumoniae producing KPC enzymes.Methods:(1) Totally846strains of Klebsiella pneumoniae were retrospectively analyzed by clinical laboratory dissociation from the first affiliated hospital of Shanxi medical university of2013. The VITEK-Ⅱ of Biomerieux were used to identified the846strains. Drug sensitivity test was performed by K-B method of CLSI. WHONET5.6software was used to analyze the data.(2)23strains of multidrug resistant Klebsiella pneumoniae were collected from the first affiliated hospital of Shanxi medical university of2013. PCR was used to detect blaKPC gene. KPC-2enzyme sequence comparisons were made to ATCC17978(GenBank accession no. NC009085) in GenBank.Results:(1) Analysis by WHONET5.6software. In the846strains of Klebsiella pneumoniae,23strain resisting3strains on existing clinical imipenem, meropenem-resistant is noted, besides β-lactam antibiotic which resistant rate is100%, quinolones antibiotic resistant rate is100%.Mainly distributed in neurosurgery, ICU, respiratory,and sputum samples have the highest separation rate which is66.61%.(2) In the23strains of multidrug resistant Klebsiella pneumoniaei,KPC enzyme positive rate was34.8%, coincidence rate was96%with ATCC17978in Genbank.Conclusion:(1)The situation of Klebsiella pneumoniae resistance is very seriously in the first affiliated hospital of Shanxi medical university and have spread trend. Neurosurgery, ICU, respiratory department have a high detection rate. We should strengthen clinical monitoring. Try to control the nosocomial infection and preventing the occurrence of epidemic. Sulfamethoxazoleandtigecycline, amikacin, polymyxin are the most effective antibiotic in our hospital presently.(2) We need more experiments to study and discuss others drug resistant mechanism.