Expression Of FANCD2in Colorectal Cancer And Its Correlation With Prognosis

Background and objectiveColorectal cance（rCRC）is a kind of common malignant disease which has a highincidence in the whole world[1]. In recent years, FA pathway is getting more attentionas a model of DNA cross-linking damage repair pathway. FA patients are predisposedto tumors including head and neck cancer, breast cancer, bladder cancer, non-smallcell lung cancer and so on. More and more experiments confirmed that FA pathwayparticipated in the occurrence and development of these cancers[2-5]. However, thereis a lack of agreement about the correlation between FA pathway and the prognosis ofcolorectal cancer. This study was to investigate the expression of FANCD2incolorectal cancer and its association with prognosis.MethodWe used qRT-PCR and immunohistochemistry to detect FANCD2mRNA andprotein expressions in colorectal cancer and its adjacent non-tumor tissues, and thenthe correlations of FANCD2expression and patients’ clinicopathologicalcharacteristics were analyzed.93patients with CRC who were diagnosed at The FirstPeople’s Municipal Hospital of Guangzhou from2008.01to2009.04and had theintegrity of clinical data and return data were retrospectively included in this study.Immunohistochemistry was used to detect the expression of FANCD2protein in all93specimens. Combined with patients’ clinical data, the correlation betweenFANCD2expression and patients’ prognosis were analyzed. Software SPSS13.0wasused for statistical analysis; the Kaplan-Meier method and log-rank test carried on the single factor analysis; Cox proportional hazards models applied to multi-factoranalysis, logistic regression models for lymph node metastasis; P <0.05wasconsidered statistically significant.Result1. The results of qRT-PCR: FANCD2mRNA both expressed in colorectalcancer tissue and in those its adjacent non-tumor tissues,0.102(0.047,0.047) versus0.051(0.025,0.025), statistically significant (P=0.007). The high levels ofFANCD2mRNA expression were positively related with lymph node metastasis andDukes Ⅲ/Ⅳ, whichwas not correlated with gender, age, degree of differentiationand position.2. The results of immunohistochemistry: the level of FANCD2proteinexpression in colorectal cancer and its adjacent non-tumor tissues were4.82±1.86versus1.57±0.98, statistically significant P＜0.0001. The high expression levelswere positively related with lymph node metastasis and Dukes Ⅲ/Ⅳ.3. The results of Kaplan and Meier survival analysis: the survival times in thegroup of FANCD2high expression, lymph node metastasis and distant metastasiswere obviously decreased; the differences were statistically significant.4. Cox multiple factors showed that distant metastasis was an independentfactor affecting survival prognosis. Logistic regression models showed thatFANCD2protein expression was closely associated with lymph node metastasis.Conclusion1. FANCD2mRNA and protein highly expressed in colorectal cancer and thehigh expression levels were positively related with lymph node metastasis andDukes Ⅲ/Ⅳ.2. The survival times in the group of FANCD2high expression, lymph nodemetastasis and distant metastasis were obviously decreased; the expression ofFANCD2protein was closely associated with lymph node metastasis; FANCD2mayhave a certain ability in the future to predict prognosis.