Font Size: a A A

Association Between Plasm PAI-1Levels And Clock Genes Polymorphisms In Primary Hypertensions

Posted on:2015-08-26Degree:MasterType:Thesis
Country:ChinaCandidate:J Y LiFull Text:PDF
GTID:2284330422987890Subject:Internal Medicine
Abstract/Summary:PDF Full Text Request
Objective: To explore the relationship between plasm PAI-1levels and Clock T3111C&Bmal1A1420G genes polymorphisms in patients with primary hypertension.Methods:334essential hypertension patients who never take antihypertensivemedications or stop the medications for at less a week were selected from October2011to January2014in the Department of Cardiovascular Diseases of FujianProvincial Hospital. All the subjects were divided into two groups based on themedian of plasm PAI-1levels (55.83ng/ml): high PAI-1group (n=167) and controlgroup (n=167). Ambulatory blood pressures, anthropometric measurements andbiochemical indicators were recorded. Polymerase chain reaction (PCR) was used tocharacterize Clock genotypes (T/C); PCR and restriction fragment lengthpolymorphism (RFLP) was employed to characterize Bmal1genotypes (A/G). ELISAwas used to detect Plasm PAI-1levels.Results:(1) Clinical characteristics: Compared with control group, high PAI-1group hadhigher waist circumferences, body mass index (BIM), triglyceride (TG), insulin,insulin resistance index (IR) and night systolic hypertension incidence (P<0.05), hadlower high-density lipoprotein cholesterol (HDL-C)(P=0.008). There were no groupdifferences in ages, sex, total cholesterol (TC), low-density lipoprotein cholesterol(LDL-C), fasting blood glucose pressure (FBG), glycohemoglobin, ambulatory bloodpressure monitoring data, sleep disorder and night diastolic hypertension incidence (P>0.05).(2) Gene polymorphisms:①The frequency of different genotypes of Clock andBmal1were in accordance with Hardy-Weinberg equilibrium in our study (P>0.05).②Clock gene: Compared with control group, Clock gene CC and CT genotypesdistribution frequency were higher in high PAI-1group(χ2=19.67, P<0.001), and Callelic frequency was also higher in high PAI-1group(χ2=46.72, P<0.001).③ Bmal1gene: Compared with control group, high PAI-1group had higher Bmal1geneGG genotype distribution frequency(χ2=15.04, P=0.001), and also had higher Gallelic frequency(χ2=34.70, P=0.001).④Combined effects of Clock and Bmal1genes: There were four kinds of genotype combinations(CC/CT-GG, CC/CT-AA/AG, TT-GG, TT-AA/AG)according to χ2test results, and the compositions ofgenotype combinations were different between high PAI-1group and control group(χ2=30.01, P<0.001). Only Clock C allelic and Bmal1GG genotype combination(CC/CT-GG) distribution frequency were more in high PAI-1group than controlgroup by partitions of χ2method (P<0.0071).(3) Analyze risk factors of elevated plasm PAI-1: Clock and Bmal1genotypes wereindependent risk factors of elevated plasm PAI-1whereas all these combinations ofClock and Bmal1genotypes did not enter into the regression equation by logisticregression. For Clock gene, minor C allele carriers (CC/CT) had a2.5higher risk ofelevated PAI-1than did noncarriers(OR=2.494,95%CI:1.450~4.289, P=0.001). ForBmal1gene, GG genotype subjects had a2.4higher risk of elevated PAI-1than did Acarriers(AA/AG)(OR=2.386,95%CI:1.435~3.966, P=0.001). Clock and Bmal1polymorphisms had not significant interaction effects on plasm PAI-1levels.Conclusion: Clock and Bmal1genes polymorphisms were risk factors of elevatedplasm PAI-1in patients with primary hypertension, and these polymorphisms had notsignificant interaction effects on plasm PAI-1levels.
Keywords/Search Tags:PAI-1, Clock, Bmal1, genes polymorphism
PDF Full Text Request
Related items