Elevated Caveolin-1mRNA Expression Of Peripheral Blood Mononuclear Cells In Hepatocellular Carcinoma Patients

Aim：Investigated the role and expression of caveolin-1mRNA which from the peripheralblood mononuclear Cells in hepatocellular carcinogenesis patients. Try to explore thesignificance of caveolin-1mRNA in the generation, progress, metastasis, invasion,poor survival rate and prognosis of hepatocellular carcinogenesis patients.Methods:The peripheral blood mononuclear Cells （PBMNCs） were extracted from60hepatocellular carcinoma (HCC) or70health people by red blood cell lysis buffer.Caveolin-1gene expression was examined by semi-quantitative RT-PCR.Thancompare the caveolin-1mRNA expression in the HCC with normal people, the HCCgrade (localized vs advanced), histological differentiation (poorly, moderatedifferentiated vs high differentiated) and the HCC with or without (AFP,HBsAg,cirrhosis, hepatic venous invasion, and intrahepatic metastasis) positive.Result:Caveolin-1mRNA expression of the PBMNCs was significantly increased in HCCcompared with normal people (p=0.017); HCC Patients with advanced stage diseasehad higher caveolin-1expression when compared with the early stage disease(p=0.023； p=0.041); Caveolin-1expression was increased in poorly, moderatedifferentiated compared with well differentiated (p=0.041; unpaired Wilcoxon ranksum test).Conclusion：This is the first study to show that Caveolin-1mRNA expression in PBMNCs by usingquantitative real-time PCR, it is obviously higher in HCC compared withcorresponding normal specimens and increases with tumor stage. Caveolin-1mRNAexpression in PBMNCs might serve as a candidate biomarker for objective prognosis indicating HCC aggressiveness. Our data encourage further investigations todetermine the role of Caveolin-1in HCC.