| Objective Build the food allergic models with SD young rats for ovalbumin(OVA)through sensitized by intraperitoneal injection(i.p) and challenged by gavage，to explorethe effect of montelukast on inflammatory of the intestinal mucosa and serum OVA-IgE infood allergy rats.Methods32female Sprague-Dawley(SD) young rats (three weeks old) wererandomly divided into4groups: the Non-Model Non-Intervene group (M0I0)、Non-ModelIntervene group(M0I0)、Model Non-Intervene (M1I0) and Model Intervene group(M1I1),8in each group. SD young rats in M1I0、M1I1groups were sensitized by intraperitonealinjection(i.p)with0.2mL of0.2mg mL-1ovalbumin solution in normal saline injected ondays0,2,4,7,9,11. To potentiate the immune response,5mg mL-1AL(OH)3adjuvantmixed with0.2mL of0.2mg mL-1ovalbumin solution was injected on day0. The youngrats were challenged by gavage with2.0mL of15mg mL-1ovalbumin solution on days20,24,28,30. M0I0、M0I1groups were i.p and gavaged with the same capacity of normalsaline instead of ovalbumin over the same period. M0I1、M1I1groups were gavaged withMK(10mg/kg·d) everyday from day20to30. M0I0、M1I0groups were gavaged with thesame capacity of normal saline over the same period. The weight growing, the appearances,the activity, the defecation of SD rats were observed by using ethology methods. Bloodwas taken in angular vein on days0,20,30. Ovalbumin specificity IgE (OVA-IgE) levelsin serum were analyzed by Enzyme-linked Immunoassay (ELISA). On day30, ratsexecuted and tissues of the two segments of jejunum were obtained, one was used toobserve the intestinal mucosa forms and Eosinophils(EOS) by Hematoxylin and Eosin (HE)Staining and using optical mircroscope, the other (fresh tissues) was used to observe mastcells(MC), account the density of MC and the percentage of integrity by frozen section andtoluidin blue staining. SPSS19.0software for Windows was used in all statistical tests. Thevalue of α=0.05was considered significant. Result (1) There was no statistical differencerespectively in average body weight between the four groups on days0,7,14,21,28,30 (P>0.05). Most SD rats in M1I0group display bristling and lusterless, seldomly moving,restlessness or diarrhea after challenge. And symptoms in M1I1group above were better.None of that above happened to M0I0and M0I1groups. In M1I0group,the intestinal mucousmembrane of jejunum were damaged to some extent. The damage in M1I1group was lessthan M1I0group. The intestinal mucous membrane of M0I0and M0I1groups were complete.(2) Before sentizied: There was no statistical difference in serum IgE(μg/ml) between thefour groups. M0I0(6.28±0.61), M0I1(6.32±0.59), M1I0(6.16±0.71), M1I1(6.51±0.64),respectively. Before challenge: The serum IgE(μg/ml) in M1I0and M1I1groups was higherthan M0I0and M0I1groups(P<0.05). M0I0(6.80±0.69), M0I1(6.56±0.63), M1I0(9.75±0.65), M1I1(9.75±0.62), respectively. After challenge: The serum IgE(μg/ml) in M1I0group was higher than M0I0, M0I1and M1I1groups(P<0.05). M0I0(7.01±0.61), M0I1(6.66±0.61), M1I0(13.12±0.70), M1I1(10.19±0.70), respectively. The density of EOS(/HPF)were M0I1(8.4±1.9), M1I0(26.4±1.8), M1I1(16.4±2.2), respectively. The density of MCwere M0I0(2.3±0.4), M0I1(2.3±0.4), M1I0(13.5±0.7), M1I1(4.3±0.6), respectively. Thepercentage of integrity were M0I018.3(16.7,23.3)%, M0I119.1(17.0,22.9)%, M1I076.8(76.0,76.8)%, M1I145.1(43.0,48.6)%, respectively. The density of EOS, the densityof MC, the percentage of integrity in M1I0group were higher than M0I0, M0I1and M1I1groups(P<0.05)；(3) By factorial ANOVA, two factors of model and montelukastintervention had taken effect on the serum OVA-IgE、the density of EOS、the density ofMC and the percentage of integrity.There were interaction between model and montelukastintervention.Conclusion Montelukast could release the symptoms of FA and the gastrointestinalinflammation effectively. It suggested that the mechanism may relate with decreasing theconcentration levels of OVA-IgE in serum, reducing the number of EOS, MC, thepercentage of MC degranulation in jejunum. |
PDF Full Text Request