Study On The Mechanism Of Thyroid Hormone Regulating AGRP Expression Through MTOR In The Hypothalamus

Objective:1. Study on the effect of thyroid hormone on AGRP expression in thehypothalamic GT1-7neuron cells.2.Explore the mechanism of thyroid hormoneregulating AGRP expression through mTOR pathways.Method:1. GT1-7cells were treated by T3in different concentrations and differenttime. The levels of AGRP mRNA expression were detected by Realtime PCR.2.GT1-7cells were treated by T3with or without PI3K inhibitors LY294002ormTOR inhibitors rapamycin separately. Then，the levels of AGRP mRNA expressionwere detected by Realtime PCR.3. GT1-7cells were treated by T3with or withoutPI3K inhibitors LY294002or mTOR inhibitors rapamycin separately. The levels ofAGRP protein expression were detected by Western Blot.Result:1. Expression of AGRP mRNA increased after GT1-7cells treated by T3indifferent time (12-72hours) and concentrations (160-800nmol/L).2.The levels ofAGRP mRNA expression were highest in GT1-7cells treated by T3in320nmol/Lafter24hour.3. Expression of AGRP mRNA was inhibited by PI3K inhibitorsLY294002and mTOR inhibitors rapamycin.4. Expression of AGRP protein wasalso inhibited by PI3K inhibitors LY294002and mTOR inhibitors rapamycin.Conclusion: Thyroid hormone can increase expression of AGRP mRNA and proteinin the hypothalamic GT1-7neuron cells, which may be through the mTOR signalingpathways.