Influence Of Bacteroides Thetaiotaomicron On Blood Glucose In Rats And Relationship Between Hyperglycemia And Atherosclerosis Disease In Diabetic Rats

Part1Influence of Bacteroides thetaiotaomicron on blood glucose in ratsAbstractObjective:To explore the effect of Bacteroides thetaiotaomicron (BT) on the level of fasting blood glucose (FBG) in rat.Methods:SD rats were randomly divided into the ordinary feed group (group A), the high-fat feed group (group B), the high-fat feed+BT filling and feeding group (group C), the high-fat feed+diabetes model control group (group D) and the high-fat feed+diabetes model+BT filling and feeding group (group E). The type2diabetes model in rat swas prepared using streptozotocin (STZ) via intraperitoneal injection. The BT bacteria liquid was fed in rats of group C and group E at the experimental first week once a day for seven days. The level of fasting blood glucose in serum was detected using hexokinase method at the zero, fourth, eighth and twelveth week. Fasting insulin (FINS) in serum was examined using ELISA method. The amount of BT in intestinal tract in rats were determined using Real-time PCR. The body weight of rats was recorded once a week.Results:(1) The amount of BT in intestinal tract in rats were significantly increased in the D and E groups compared with the A, B and C groups(P<0.01), in which the amount of BT were more in the C group than in the B group (P<0.01), and in the E group than in the D group (P<0.01).(2) The body weight of rats was significantly increased in the C group compared with the B and D groups (P<0.01), and in the E group compared with the D group (P<0.01).(3) The level of fasting blood glucose in serum in rats was significantly increased in the C group compared with the A and B groups from the eighth week to twelveth week (P<0.01), and in the E group compared with the D and C groups from the eighth week to twelveth week (P<O.01).(4) In rats, the amount of BT in intestinal tract were positively correlated with body weight (r=0.163, P<0.05), with the level of fasting blood glucose (r=0.684, P<0.01), and were no correlated with Fasting insulin in serum (r=-0.019, P>0.05).Conclusion:The Bacteroides thetaiotaomicron in intestinal tract not noly promotes increase in body weight, but also promotes increase in blood glucose in rats. Part2Relationship between hyperglycemia and Atherosclerosis Disease in Diabetic RatsAim:To explore the relationship between hyperglycemia and atherosclerosis disease in diabetic rats.Methods:The models of diabetic ratswere made by injecting streptozocin into rat.s abdomen. Wistar rats were divided into four groups:three diabetic groups and one control group. At one month, the third month and the fifth month, the rats.arterys were collected as experimental material to observe atherosclerosis and cholesterol, triglyeride, high-t density lipoprotein, low-density lipoprotein, plasma glucose homocysteine, fructosamine were examined.Results:cholesterol,triglyeride, low-density lipoprotein, plasma glucose, homocysteine, fructosamine level in diabetic group were higher than that in the control group and we could observe that there were typical characteristics of atherosclerosis in the diabetic rats.Conclusions:The diabetic rats model which only have hyperglycemia have atherosclerotic disease.