| Sweet calabash (Lagenaria siceraria (Molina) Standl.var.hispisa (Thund.Hara) is an annualprostrate herb, also known as "meat dish calabash gourd""the flower" Japan called dried gourd,is a kind of gourd melon, it has good medicinal value and edible value. In daily life, people tookSweet Calabash fresh thick flesh as edible parts, it has a high nutritional value, the main reasonis that contains a variety of nutrients, if sugar, glucose, vitamins, calcium, potassium,phosphorus and other elements, plant secondary metabolites of Sweet Calabash also contains ahighly oxidizing important the cucurbitacin, cucurbitacin belongs to the city of Victoria threeterpenoids, according to a survey of medical researchers are using its research TolperisoneHydrochloride Tablets, gourd into capsules, its main function is the anti-cancer, the productsalso have a certain effect on high blood pressure, high blood fat, arteriosclerosis, diabetes andother diseases. In the ecosystem, a variety of animal and plant pathogenic bacteria feedingwould infringe Cucurbitaceae plants, but this time as a heterologous semiochemicalscucurbitacin can effectively avoid the damage effect. In addition, cucurbitacin can promoteimmune function of cells, with jaundice, ascites and other biological activity of scavenging andinhibiting the proliferation of liver fibrosis.China has abundant resources of Sweet Calabash, sweet calabash and use of the resourcesin order to better development, this study in order to improve the yield as the objective, tocucurbitacin B and cucurbitacin E as the research object, the ultrasonic assisted extraction, wasisolated and purified by macroporous resin, and the use of cucurbitacin B and block based on thebasis of E antibacterial test. The results are as follows:1.The optimum extraction conditions of cucurbitacin B and cucurbitacin E were obtained bysingle factor test and orthogonal test as follows: the ratio of material to liquid was1:10, thesolvent concentration of70%, ultrasonic time15min, ultrasonic power500W. The optimumextraction conditions of extraction of cucurbitacin B and cucurbitacin E, get the cucurbitacin Byield is0.799%and cucurbitacin E yield is0.695%.2.Through static adsorption test by4kinds of macroporous resin, get the optimal resincucurbitacin B and cucurbitacin E is XAD-16;get the cucurbitacin B reached equilibrium after5h, and cucurbitacin E after4h reached the stable equilibrium state.Dynamic adsorption dynamics by macroporous resin for purification of cucurbitacin B andcucurbitacin E test, the optimal dynamic macroporous resin obtained to cucurbitacin B adsorption kinetics experiment conditions for the sample flow rate is3mL/min, the elutionvelocity is2mL/min, the eluent concentration is50%; the optimal dynamic macroporous resinobtained to cucurbitacin E adsorption kinetics experiment conditions for the sample flow rate is3mL/min, the elution velocity is3mL/min, the eluent concentration is50%.The optimum purification conditions obtained by dynamic adsorption and desorptionexperiments of macroporous resin purification test of cucurbitacin B and cucurbitacin E,purified product of cucurbitacin B reached68%, cucurbitacin E reached14%, indicating thatXAD-16macroporous resin purification of cucurbitacins effect is good.3. Through the antibacterial test of cucurbitacin B and cucurbitacin E on Escherichia coli,Saccharomyces cerevisiae, Aspergillus niger, determine cucurbitacin B and cucurbitacin E hascertain inhibitory effect on Escherichia coli, Saccharomyces cerevisiae, Aspergillus niger.Cucurbitacin B average zone of inhibition on Escherichia coli, Saccharomyces cerevisiae,Aspergillus niger diameter are10.9mm,8.1mm,10.4mm; the minimum bacteriostasisconcentration range are0.0625g/mL-0.125g/mL,0.0625g/mL-0.125g/mL,0.125g/mL-0.25g/mL.Cucurbitacin E average zone of inhibition on Escherichia coli, Saccharomyces cerevisiae,Aspergillus niger diameter are11.3mm,9.5mm,8.7mm; the minimum bacteriostasisconcentration range are0.0625g/mL-0.125g/mL,0.03125g/mL-0.0625g/mL,0.25g/mL-0.5g/mL. |
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