The Feasible Studying Of MCV And MCH In Screening Thalassemia From Neonatal Cord Blood Erythrocyte

【Purpose】Discuss the feasibility of screening for thalassemia using neonatalcord blood MCV and MCH in the Guangdong area，to determine the optimalcut-off value of MCV and MCH. Grasp the thalassemia gene carrying rateof neonatal in Guangdong area.So as to achieve early detection， earlydiagnosis and early treatment of neonatal thalassemia purposes. Createan electronic health records，marriage guidance，reducing the birth ratein neonatal with thalassemia.【Methodology】During the May2012to December2012,we Selection of GuangdongProvince existing birth defects monitoring county (District) as thebaseline survey area (each pumping and1counties),then,in each of thecounty,91hospitals were selected (which the numbers of delivery weremore than600yearly), and from the hospitals above, the total qualifiedumbilical cord bloods were13396.All the blood samples using standardautomatic blood cell analyzer for blood cell analysis. Technology for genedetection by our institute with independent intellectual property rightsof the liquichip. Results of all the data unification input.Using the SPSS17.0statistical software for statistical analysis.【Result】13396cases of neonatal cord blood by gene detection， Diagnosis1571cases of αthalassemia gene carriers (accounting for11.73%)，500cases of β thalassemia gene carriers (accounting for3.73%)，76cases of αβthalassemia gene carriers(accounting for0.57%). The sensitivity ofneonatal screening for α thalassemia using MCV and MCH were84.8%and90.3%respectively，the specificity were68.9%and74.6%，the cut-offvalue were104fl and34pg; The sensitivity of neonatal screening forβ-thalassemia were80.4%and81.6%respectively， the specificity were31.2%and34.6%， the cut-off value were110fl and36pg. The sensitivityof neonatal screening for αβcompound were81.6%and90.8%respectively，the specificity were68.9%and87.8%，the cut-off value were104fl and33pg.【Conclusion】Screening and diagnosis of neonatal cord blood MCV and MCHin neonatal thalassemia has great value.Neonatal cord blooderythrocyte index of different types of thalassemia gene incomprehensive analysis，can obtain different cut-off values ofthe results， and make the reference for the clinical diagnosis ofthalassemia; there is the risk of neonatal cord blood， routine screening，then the gene detection of thalassemia in children with suspected neonatalscreening of thalassemia， provide services for the disease risk family.