Effect Of Erythropoietin On Caspase-3、GAP-43Expression And Nerve Repairment After Sciatic Nerve Injury In Rats

Objectives: To explore the effect of erythropoietin on Caspase-3andGAP-43gene expression of the relevant segment spinal marrow after sciaticnerve injury in rats and provide experimental basis for clinical treatmentof peripheral nerve injury.Methods:45Sprague-Dawley rats,male or female, were divided intothree groups by random number method: Matched group, model group and testgroup，each group has15Sprague-Dawley rats.The model of the sciaticnerve defect was set up: after disinfected as normal, the sciatic nerveinside the piriformis was exposed and clamped2times with needle holder.Every clamp lasted30seconds and the intervals were10seconds. Matchedgroup for cantrast were fed as normal, model group were injected withnormal saline and test group were injected erythropoietin respectively.At1w,2w, and3w after operation,15rats from each group were taken forthe detection of sciatic nerve function index,the histopathologyobservation of the injured nerve and the expression of caspase-3andGAP-43of the relevant segment spinal marrow was tested with PCR method.Results: The sciatic functional index, the Matched group is normal atanytime,the modal group and the test group were reduce significantly,butsimilar at1w（P〉0.05）,the test group was better than the modal groupat2w and3w（P〈0.05）.HE staining in Matched group: the nerve tissuewas normal,and the neuronal morphology were intact,none edema andInflammatory cell infiltration； in model group:the nerve tissue wasedema, vacuolar degeneration, karyopyknosiss and Inflammatory cellinfiltration；in the test group, the pathological change of the nervetissue was mild.The expression of Caspase-3and GAP-43was low in the matched group,but increased after the nerve injured in the model groupand the test group, the difference between the model group and the testgroup was not significant before1w（P〉0.05）,while significant after1w（P〈0.05）,The expression of Caspase-3in test group was less than themodel group and the expression of GAP-43in test group was more than themodal group.Conclusions: EPO can inhibit the expression ofCaspase-3and promote theexpression of GAP-43, which may provide protective effect on sciatic nerveinjury in rats.