After Intestinal Bypass Surgery Different Food Influence Type2Diabetic Rats Blood Sugar And Lipids

【AIM】Observed after Duodenal-jejunal bypass and New Billiopancreatic diversion therelationship between the different food fed glucose and lipid changes between itsfurther explore Surgery treatment of type2diabetes may mechanisms.【METHODS】1、Purchase of4-5week-old male SD rats (SPF)60, combined with high-fathigh-sugar diet and low-dose (35mg/Kg) streptozotocin (STZ) intraperitonealinjection of construction type2diabetic rat model, random blood glucose screening≥16.7mmol/L as a type2diabetic rat model into a mold. The33successful modelof type2diabetic rats were randomly divided into A, B, C three groups. The group ofA underwent DJB surgery (12), the group of B underwent NBPD (11), on the shamsurgery group C (10).2、Detected before surgery and one week after the surgery first week of the rats1,2,4,6,8,10fasting weight, fasting plasma glucose (FPG),2-hour postprandialglucose of changes. Detected before surgery one week and two weeks after fastingserum cholesterol (Ch), fasting serum triglycerides (TG), fasting serum free fattyacid (FFA) and postprandial serum cholesterol, triglycerides and change of free fattyacids.3、15-20days after the first order, respectively, to20%in long-chain fat milk,peanut oil press8kcal/kg orally, intravenously0,120minutes canthus serumcholesterol, triglycerides and free fatty acids in the blood test.21-26days after theorder, respectively, glucose, starch8kcal/kg gavage, tail vein blood glucosemeasurements0,30,60,120,180minutes, and calculate the change in area under thecurve (AUC); postoperative27-29to Ansul by8kcal/kg gavage,0,120minutes todetect glucose and serum cholesterol, triglycerides and free fatty acids changes andadded measure minute changes in blood glucose30,60,180 【RESULTS】1、After surgery three groups of rats were elevated body weight, and2weeksafter surgery DJB group and the sham difference was statistically Count significance(P <0.05), after surgery4Wednesday rat body weight were not statistically significant(P>0.05), after surgery6weeks of the fastest growing NBPD body weight,compared with the sham and DJB group differences were there was statisticallysignificant (P <0.05), until after the first10weeks still significant differences, whileDJB group and the sham group difference was not statistically significant.2、After surgery the rats had no statistically significant difference in fastingplasma glucose (P>0.05), starting from the first week after surgery,the group ofNBPB and DJB2h postprandial blood glucose were improved, and sham surgerywith preoperative phase than the differences were statistically significant (P <0.05),up to10weeks after2h postprandial blood glucose is still statistically significant (P<0.05).3、Starch and glucose fed rats whose blood were elevated, DJB group comparedto the sham group and NBPD significant difference (P <0.05), no significantdifference between the sham group and NBPD (P>0.05), and their blood sugar spikesafter intragastric glucose was significantly higher than starch, glucose changes aftergavage with Ansul starch roughly the same.4、After surgery fat milk and peanut fed three groups of rats FFA, TG wereelevated, DJB and NBPD groups was statistically significant (P <0.05) differencecompared with sham, but there was no statistical significance between the two groups(P>0.05). And the fat emulsion administered FFA, TG and TC were higher than thepeak after peak peanut oil gavage, lipids without much change after Ansul gavage,there were no significant (P>0.05) and between various groups before surgery.5、After surgery the group of sham than before fasting FFA increased, comparedwith the preoperative difference was statistically significant (P <0.05), NBPD andDJB groups showed no significant difference (P>0.05) compared with thepreoperative. No statistically significant differences among the three groups (P>0.05).Three groups of rats fasting TG and TC twenty-two no statistically significant difference compared (P>0.05).6、After surgery the group of NBPD and DJB the postprandial FFA, TG and TCdecreased significantly compared with the preoperative compared with sham surgeryand preoperative differences were statistically significant (P <0.05), and between noStatistically significant (P>0.05).【CONCLUSION】1、Duodenal-jejunal bypass (DJB) and New Billiopancreatic diversion (NBPD)can reduce postprandial blood glucose in type2diabetic rats.2、DJB and NBPD group, because pancreatic bile mixed with food delays canaffect the digestion of carbohydrates, DJB group due to the duodenum and proximaljejunum exclusion may reduce intestinal absorption of saccharides which may is oneof its hypoglycemic mechanism.3、DJB and NBPD group can affect the digestion and absorption of lipids, lipidsand thus reduce the toxicity of the pancreas, improving insulin sensitivity, therebyimproving blood sugar, may be one of its hypoglycemic mechanism. The mainimprovement of lipid is due to bile and pancreatic bypass.