| Seabuckthorn has a variety of nutrients and many kinds of biological activities andpharmacological effects, therefore functional food or medicine are developed with theSeabuckthorn seed oil as raw material is growing, but there are no unification qualitystandards for the control of Seabuckthorn seed oil soft capsule’s quality. Seabuckthornseed oil soft capsule is health care function of immunity isenhanced by seabuckthornseed oil, ginseng extract, vitamin E and othermain effective components. This subjectestablish the detection method of the main active constituents containing totalginsenoside ginseng root, alpha-tocopherol, linoleic acid, linolenic acid, and itanalyses the content of the active constituent and its enhancement immune functionresearch. This research provides the basis for Seabuckthorn seed oil soft capsule’squality control and the study of the health food. The research mainly includes twoaspects:一Analysis of the active constituents in Seabuckthorn seed oil soft capsuleObjective: To establish a method to determine total ginsenoside ginseng root, alpha-tocopherol,linoleic acid and linolenic acid in Seabuckthorn seed oil soft capsule.Andusing them as indicators of quality control of Seabuckthorn seed oil soft capsule.Methods: With70%ethanol solvent, solid-liquid ratio (m/v)1:100, ultrasonicextraction and ultrasonic temperature (60℃)30min, macroporous resin columnelution, determination of vanilla-perchloric acid method at560nm absorbance values.With aldehyde ethanol solvent, solid-liquid ratio (m/v)1:40,5ml10%antiscorbuticacid and the8ml potassium hydroxide solution (1+1), saponification reaction of30min in80℃water bath heating under reflux, glacial acetic acid neutralization,constant volume, static,0.45μm filter membrane; Chromatographic column: Thermo C18（4.6×250mm，5μm）, Mobile phase: methanol; The flow rate of1.0mL/min;Column temperature30℃;Detection wave length:292nm; Sample quantity:10μL,HPLC-PDA determination of alpha-tocopherol.With petroleum ether:ethyl ether（1:1）solvent, solid-liquid ratio (m/v)1:60,ultrasonicextraction15min,nitrogen blowing,KOHmethanol solution (0.5mol/l) methyl esterification reaction of20min,constant volume,0.45μm filter membrane;Chromatographic column: DB-wax30m0.25mm; Column temperature210℃; FIDDetector temperature：280℃；Inlet temperature：250℃；Carrier gas：N2;The flowrate of0.5mL/min; Split ratio:30:1, GC determination of the linoleic acid andlinolenic acid.Results: The total ginsenoside ginseng root average recovery of Seabuckthorn seedoil soft capsule is93.7%,RSD=2.09%; alpha-tocopherol average recovery is96.39%,RSD=1.98%; linoleic acid average recovery is98.98%,RSD=1.16%; linolenicacid average recovery is98.63%,RSD=1.09%.Couclusion: The proposed method was applied to the determination of totalginsenosides ginseng root, alpha tocopherol, linoleic acid and linolenic acid content ofSeabuckthorn seed oil soft capsule, and the method has highly repeatability andselectivity,it is simple and fast.二Study on the function of enhancing immunityThrough evaluating safety of animal experiment of Seabuckthorn seed oil soft capsule,then study it’s function of enhancing immunity which include: carbon clearancetest;determination of body weight;elayed type hypersensitivity (DTH) test;murineperitoneal macrophage phagocytosis the chicken erythrocytes experiment andorgan/body weight ratio; antibody-producing cells and serum hemolysintest;ConA-induced micelymphocyte transformation experiments and determination ofNK cell activity. According to the 《health food inspection and evaluation oftechnical specifications (Ministry of Health2003)》to determine the Seabuckthornseed oil soft capsule whether having the function of enhancing immune. |
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