Study On Preparation And Properties Of Poly(ε-caprolactone)/Bioactive Glass Fibrous Scaffold

Currently, clinical treatment of skin wounds mainly includes debriding, bandaging, autologoustransplanting, allograft transplanting and artificial material transplanting, etc. However,debriding and bandaging can lead to infection and deterioration, autologous transplanting needsmore donor sources, while allograft transplanting and artificial material transplanting issuffering from low oxygen permeability, poor nutrition transportation and slow cellproliferation. Therefore, some problems need to be addressed urgently, which concludepermeability of water and oxygen, reducing fluid loss, resisting germs, mechanical propertyand biocompatibility. Thus, designing artificial skin material which possesses natural skinstructure, good hygroscopic property, high porosity, good cell compatibility as well as goodmechanical property is of importance in obtaining better wound healing effect.Polycaprolactone (PCL) has good biocompatibility and mechanical property, while bioactiveglass (BG) shows good performance in skin wound healing. Successful combination of thesetwo materials could makes full use of their advantages.In order to prepare PCL/BG composite fibrous membranes in micro-nano scale, electrospinningtechnique was employed, which is a relatively easier method for producing nanofibers. Theoptimum parameters of electrospinning were determined by comparing micromorphology offibrous membranes prepared with various parameters such as voltage, flow rate and masspercent of BG. Surface properties, porosity, tensile property and cytocompatibility of fibrousmembranes with different BG mass percent were analyzed in detail. The results show thataddition of BG could improve hydrophilicity and elasticity modulus of membranes. However,it could also decrease the rupture stress and breaking elongation of membranes. The minimumbreaking elongation of membrane could be over100%, which means that composite fibrousmembranes have good mechanical property. The porosities of fibrous membranes with differentBG mass percent are all found over90%. In addition, composite fibrous membranes have thebest performance in cell proliferation and adhesion when the BG mass percent is6%, while toohigh mass percent of BG could be bad to cell proliferation. In order to meet the demands of electrospinning, sol-gel method and template method werecombined to prepare nano-size BG with great dispersibility. Cetanecyl trimethyl ammoniumchloride (CTAC) was introduced as template. Micelle formation effect was compared underammonia with triethanolamine (TEA), which functioned as catalysts. The results show that BGparticles have better size distribution and dispersibility. Thereafter, TEA was utilized as catalystto prepare two kinds of BG, which consisted of58S and77S. The results show that77S BG hasnano-size and good dispersibility, which is potential to be inorganic component inelectrospinning.