| Objective：To discuss the relationship between the abnormal expression of β-catenin–thecentral regulatory molecule of the Wnt signaling pathways–in a variety of gastriccancer cell lines and tissues and the clinical pathological factors, especially therelationship with the prognosis of patients with gastric cancer. By detecting theexpression degree of β-catenin in gastric cancer and para-carcinoma tissues, toanalyze the correlations between the expressions with the clinical pathological factors(tumor site, presence of lymph node metastasis (LNM), tumor differentiation, TNMstage, tumor size, age, gender and invasion depth) and the relationship between5-year survival rate and the abnormal expression of β-catenin.Methods：Cell immunofluorescence and Western Blot were used to detect the expression ofβ-catenin ina varietyof gastric cancer cells (AGS、HGC-27、SGC-7901、MGC-803、BGC-803、MKN-45、BGC-823) and normal gastric mucosa cells (GES-1)(abnormalpositioning and excessive expression).76cases of patients were enrolled in this study, all of whom were undergonegastric cancer surgery and postoperative adjuvant chemotherapy in the First AffiliatedHospital of Nanchang University from June2006to June2008. Postoperativepathology was used to confirm the presence/absence of LNM, tumor differentiationand tumor size, etc. The postoperative paraffin-embedded cancer tissue specimenswere taken from the pathological department, and38cases of paraffin-embeddedpara-carcinoma tissue specimens were also collected for comparison. All of thepatients were divided according to the pathological staging (period I and II, period IIIand IV), stages, the presence of LNM, tumor differentiation (low differentiation,medium and high differentiation), invasion depth (inside serosa, serosa and outsideserosa), tumor size (diameter≥5cm,<5cm), age (<60years old,≥60years old),gender (male, female),5-year survival rate (≥5years,<5years), etc. All of thepatients had never received any treatment (radiation, chemotherapy, interventional therapy and targeted therapy, etc.) related to the tumor before surgery, but were giventhe5-fu-based chemotherapy for more than4weeks after operation. Two stepimmunohistochemistry (IHC) was used in this study to detect the expression ofβ-catenin in gastric cancer and para-carcinoma tissues at the same time. And therelationship between the expression of β-catenin and the clinical pathological factors(tumor site, invasion depth, presence of LNM, tumor differentiation, tumor size, age,and gender and TNM stage) as well as the relationship between5-year survival rateand the positive expression of β-catenin were analyzed. SPSS18.0was used for dataanalysis. Chi-square test was used to test the relationship between expression ofβ-catenin in the gastric cancer and the clinical pathologic factors. P≤0.05was ofstatistical significance.Results：1. β-catenin is over-expressed in the gastric cancer cells AGS, HGC-27andMGC-803, and accumulated abnormally in the cytoplasm/nucleus; gastric cancer cellsMKN-45and normal gastric mucosa have lower expression, and are only expressedon the cytomembrane.2. Positive expression rate of β-catenin protein is81.6%in gastric cancer tissue,and50%in para-carcinoma tissues; Strong expression rate of β-catenin protein is61.8%in gastric cancer tissue, and23.7%in para-carcinoma tissues. There weresignificant differences of β-catenin expression between gastric cancer tissue andpara-carcinoma tissues (P <0.05), as shown in Tab.1and2.3. For over-expression of β-catenin in gastric cancer tissue, the expression ofβ-catenin is related with the tumor differentiation degree (X2=15.414, P<0.01), tumorinvasion depth（X2=3.739，P=0.048）, and tumor site（X2=4.41，P=0.031）; β-cateninhas nothing to do with the patient’s gender（X2=0.01，P=0.585）, age（X2=0.502，P=0.319）, tumor size（X2=0.173，P=0.428）, TNM staging （X2=1.771，P=0.139）and the presence/absence of LNM（X2=1.306，P=0.187）, as shown in Tab.3.4. Positive expression of β-catenin in gastric cancer tissue is significantlyassociated with postoperative5-years survival rate, as shown in Tab.4.Conclusions：β-catenin is over-expressed in multiple strains of gastric cancer cell lines, and appeared cytoplasmic/nuclear ectopic; Abnormal expression of β-catenin in gastriccancer tissue can reflect the development degree and the differentiation of gastriccancer. β-catenin over-expression plays an important role in gastric cancerdevelopment. β-catenin detection has important clinical value guiding treatment, andβ-catenin can be used as diagnosis index to evaluate the prognosis of gastric cancer. |
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