Study On Inhibitory Effects Of Resveratrol On Human Ovarian Ancer Cell Line Skov3by Downrefulating Sirt1Expression

Objective：TO study the effects of Resveratrol (Res) on the proliferation of human ovariancancer cell line Skov3and the expression of Sirt1. To investigate the relatedmechanism of Res on the growth inhibition of human ovarian cancer Skov3cells andto provide experimental and theoretical basis for the treatment of ovarian cancer.Methods：Different concentrations (0,5,10,20,40,80μg/ml respectively) of Res wereadded to the medium and Skov3cells were further cultured for24,48and72hours,MTT assay was used to detect the proliferation effects of different concentrations ofRes on human ovarian cancer Skov3cells. RT-PCR and Western Blot assay were usedto measure the different levels of Sirt1mRNA and protein expression in Skov3cellswhich were processed by different concentrations of Res after24h.Results：1. Different concentrations of Res (0,5,10,20,40,80μg/ml) acting on humanovarian cancer Skov3cells for different times (24h,48h,72h), The growth ofhuman ovarian cancer Skov3cells was restrained and the inhibitory rate wasincreased in time-and dose-dependent. There are significance difference betweendifferent groups and between each group and the control group (P <0.05);2. Different concentrations of Res (0,5,10,20,40,80μg/ml) were used toprocess human ovarian cancer Skov3cells for24h. With the increase of drugconcentration, the expression of Sirt1mRNA in human ovarian cancer Skov3wasdown regulated (P <0.05);3. Different concentrations of Res (0,5,10,20,40,80μg/ml) were used toprocess on human ovarian cancer Skov3cells for24h. With the increase of drugconcentration, the expression of Sirt1protein in human ovarian cancer Skov3wasdown regulated (P <0.05); Conclusion：1. Res can inhibit the proliferation of human ovarian cancer Skov3cells, and theinhibitory effect is in time-and dose-dependent.2. Res may supress the proliferation of human ovarian cancer Skov3cells bydown-regulating the expression of Sirt1in these cells.