Expression Of HEPN1and MMP-9and Their Relationships Correlate With Invasion In Pituitary Adenomas

Objective： To detect the expressions of hepatocellular carcinoma, down-regulated1(HEPN1)and matrix metalloproteinase-9(MMP-9)in non-invasive andinvasive pituitary adenomas,and discuss their correlations with invasion andrelationships with each other.Methods：A total of16cases of surgically resected pituitary adenoma specimenswere collected during the period of September2013to December2013at theDepartment of Neurosuegery,The First Affiliated Hospital of Nanchang University,Nanchang,China.there were7non-invasive and9invasive pituitary adenomas,whichwere defined on the modified Hardy,s classification. To detect the mRNA expressionsby reverse transcription PCR and to detect the protein expression by western blot ofHEPN1and MMP-9in7non-invasive and9invasive pituitary adenomas specimens.Result：The mRNA and protein expression levels of HEPN1were significantlylower in invasive group than in non-invasive group(P<0.05).The mRNA and proteinexpression levels of MMP-9were significantly higher in invasive group than innon-invasive group(P<0.05).The expression of HEPN1has negative correlation withthe expression of MMP-9in invasive pituitary adenomas.Conclusion：The expression of HEPN1and MMP-9are closely correlated withaggressive pituitary adenoma behavior，The down regulated expression of HEPN1may cause the rise expression of MMP-9and promote tumor invasion.