The Effects Of Berbamine On β-catenin Signal Pathway Of Jurkat Cells

Objective：To investigate the expression of β-catenin in Jurkat cells on the effect ofberbamin.Methods：The MTT assay was used to detect inhibition rate of Jurkat cells and calculatethe IC50.Flow cytometry was used to detect cell apoptosis rate. The chang ofexpression of β-catenin in Jurkat cells was analyzed through western blot. How theposition of β-catenin change in Jurkat Cells was studied after the effect of berbamine.Result：1.MTT tests revealed that bebamine inhibited Jurkat cells in dose-andtime-dependent manner.The IC50values of berbamine were20.75μg/ml、10.32μg/ml、6.75μg/ml for24h、48h and72h.2.The apoptosis rate of Jurkat cells detected by flow cytometry instrument for3h、12h and24h were7.1%、23.4%、66.8%, increased with the extension of time.3.Western blot revealed that expression of β-catenin in Jurkat cell reduced afterthe effects of bebamine.4.The immunofluorescent assay Prompted that the nuclear translotion ofβ-catenin reduced after the effects of bebamine.Conclusion：1.Bebamine could inhibit the proliferation of Jurkat cells and induce apoptosis indose-and time-dependent manner.2.The proapoptotic effect of bebamine on Jurkat cells may be achieved by downregulating the expression of β-catenin protein.3.Bebamine could inhibit the proliferation of Jurkat cells through inhibiting thenuclear translotion of β-catenin.