| Maillard reaction means the non-enzymatic browning reaction between the reduced sugarand amino acids at heating condition. Today it shows potential as a diabetes, obesity, mutationprophylaxis and treatment. The ginseng has the arginine and maltose which have a Maillardreaction when the fresh ginseng is prepared to be the red ginseng. The AFG it generated was thereason that the red ginseng’s efficacy was different from the white ginseng’s. In this paper, theAFG the composition, purification and pharmacokinetics were researched.This paper built the process of purifying the AFG in combining cation exchange resin withpolyacrylamide gel column chromatographic method. The the crude product of AFG wasappended to the cation exchange resin column with1/5times of resin volume and eluted by thedistilled water to a neutral stance. Washing with4%ammonia and using TLC for tracking theAFG. Collecting the component which value of Rf was0.156and removing the ammoniumhydroxide. After lyophilization the powder was diluted to Bio-gel P-Ⅱ of0.5g mL-1by distilledwater. Collecting the pure component and receiving the AFG which purity was98.6%. Theresults showed the fast separation speed and large amount of enrichment.The object of this study was the pharmacokinetics of AFG in rats. In this experiment, therats’ blood was collected at the different time by design after oral administration(50mg kg-1,25mg kg-1,10mg kg-1). The precolumn derivatization was used in measuring the bloodconcentration, distribution and excretion. The parameters of pharmacokinetics were computedwith3P97software. After carrying on the data fitting to the experimental findings with3P97software, pharmacokinetics of AFG were fitted to a three-compartment model. There was nosignificant difference in Tmax among three dosage groups. The t1/2in the lowest group wassignificantly different with the other groups. In lower range of doses(10mg kg-1~25mg kg-1),there was a linear relationship between AUC and the dose，when reached50mg kg-1，the AUCincreased significantly. After oral administration(50mg kg-1), the distribution of drug in1,2and4hours was liver> kidney> spleen> stomach> lung, muscle> small intestine> testis,heart> brain> uterus> fat. The result of the excretion test showed that the drug excreted inurine accounts for roughly (4.31±0.06)%of dose in48hours, the drug excreted in faecesaccounts for roughly (0.16±0.03)%of dose in48hours, the drug excreted in bile accounts forroughly (3.31±0.14)%of dose in24hours. Through the low, medium and high doses of t1/2(5.2±0.1,4.6±0.1,4.7±0.1)h, we knew that different doses in the process of AFGpharmacokinetic in rats had differences, and the AFG eliminated faster in rats, no accumulation.The AFG would break down into AF or other styles and mainly be excreted in urine. We can learn by experiments that the AFG which was got by isolating and purifying hadthe good linearity in rats fitting to the three compartment model. The drug was absorbed fast andwas not possibly easy to accumulate in the body. The tissue distribution and excretion pathwaywere obvious. |
PDF Full Text Request