| 【Objectives】To study the inhibiting effect of curcumin, vitamin C of different concentration andcurcumin-vitamin compound to gastric cancer cell SGC-7901as well as the effect toperiodic apoptosis. To discuss the50%inhibiting concentration of curcumin, vitamin Cin gastric cancer SGC-7901cells at different time and the effect of the combined effectsof the two drugs. In order to find new chemotherapy drugs, chemotherapy plan, and theappropriate concentration to further improve the effect of gastric cancer chemotherapy inclinical treatment and reduce the adverse reaction of chemotherapy on the basis ofexperiment.【Methods】1. When curcumin (5,10,20,40umol/L), vitamin C (0.25,0.5,1.0,2.0mg/mL) ofdifferent concentration and curcumin-vitamin compound were put into use, MTTcolorimetric assay was adopted to detect the growth of Gastric cancer cell SGC-7901respectively after24,48and72hours.Using statistical program to analysis IC50inhibiting concentration of different time. Through calculation results analysis the twodrug combination effect.Then use inverted microscope to observe the change of cellnumber and form of cell SGC-7901after48hours.2. Detect the effect that SGC-7901would bring to cells cycle when curcumin (20,40umol/L), vitamin C (1mg/mL) and curcumin-vitamin C compound acted for48hoursby adopting PI color-flow cytometricassay.3. Use AnnexinV-Pl color-flow cytometricassay to detect apoptosis rate of cellSGC-7901when curcumin (20,40umol/L), vitamin C (1mg/mL) and curcumin-vitamin C compound acted for48hours. At last, use fluorescence microscope to detect cell’sapoptosis form when curcumin (20,40umol/L), vitamin C (1mg/mL) andcurcumin-vitamin C compound acted for48hours.【Results】1. Proliferation inhibiting function was shown when Curcumin (5,10,20,40umol/L)acted to gastric cancer cell SGC-7901for24,48and72hours respectively. Furthermore,as the concentration became higher and acting time became longer, the inhibiting ratealso enhanced, which was proved by dependency on time and dose. After the action ofvitamin C of different concentration, the inhibiting function also enhanced gradually asthe concentration increased; however, the inhibiting rate had no obvious change as thetime went by. When these two drugs worked together, the inhibiting rate increasedobviously. Through calculation results the joint effect of the two drugs was their additionafter24h, synergy besides the highest concentration of combined the two drugs togetherafter48h,72h.Through inverted microscope, it was observed that as the increase ofconcentration of each medicine, the number of dead and cast-off cells also increased;When these two drugs worked together, the number of dead and cast-off cells increased.2. After curcumin (20,40umol/L), vitamin C (1mg/mL) and curcumin-vitamin Ccompound acted on cell SGC-7901for48hours, it was found that they could arrest cell(SGC-7901) cycle in phase G0/G1compared with Negative control group. While it wasobvious that high concentration curcumin group and combined high concentration grouparrested larger proportion of cells.3. After curcumin (20,40umol/L), vitamin C (1mg/mL) and curcumin-vitamin Ccompound acted on cell SGC-7901for48hours, both drugs could induce apoptosiscompared with negative control group. The result showed that apoptosis rate of groupcurcumin is much higher than that of the combined group, and the combined group washigher than that of individual group. This is of significance for statistics (P<0.05). It wasobserved with fluorescence microscope that the number of apoptosis cell in group curcumin increased with the increase of concentration. Compared with negative controlgroup, the number of apoptosis cell in group Vitamin C apparently increased. Besides,the combined group has more apoptosis cells than that in individual medicine group.【Conclusion】Both curcumin and vitamin C can inhibit the proliferation of gastric cancer cellSGC-7901. Within certain concentration range, with the extension of time and theincrease of the concentration, the proliferation inhibition of Curcumin enhanced; whilefor vitamin C, only proliferation inhibition increased with the increase of concentration,there is no obvious relation with time prolonging. When two medicines are combined, theeffect of inhibition is much better than that of individual medicine. When two medicinesjointly act for24h, the effect is equal to that of two medicines; while when two medicinesjointly act for48h or72h, the effect is better than that of two medicines. Either individualmedicine or combined could arrest cell cycle in G0/G1phase. The combined group canarrest more cells than individual medicine group; what’s more, the combined medicinegroup can induce apoptosis much more effective than individual medicine group. Thisprovides the experimental basis for applying two medicines combination into clinicalchemotherapy of gastric cancer. |
PDF Full Text Request