Research Of CIK Cells In The Treatment Of Nasopharyngeal Carcinoma And Its TLR Expression

Objective: We observe the recent clinical efficacy of CIK cells in the treatment ofnasopharyngeal carcinoma, and study the experimental of Toll-like receptors mRNA(TLR1～TLR10) in CIK cells, further elaborated the anti-tumor mechanism of CIK cells,evaluation of CIK cells clinical adoptive immunotherapy the feasibility of treatment.Methods:1. Select qualified diagnosed late20cases of previously untreated patients withnasopharyngeal carcinoma, they are divided into the experimental group (radiotherapy+chemotherapy+CIK therapy) and control group (radiotherapy and chemotherapy)according to age, sex, and treatment methods. Observed the two groups of patients withdifferent treatment quality of life, physical condition (diet, sleep and weight) andapplication of SDS ADS were evaluated mental status before and after treatment of twogroups of patients, at the same time, observing the clinical response of CIK cells aftertransfusion. Differences in the quality of life and psychological status of patients before andafter treatment with paired t test.2. Extract the venous blood of healthy volunteers, Ficoll separation was centrifuge toseparate the PBMC, reference standards of reporting CIK cell culture method training CIKcells generate.3. In3day,6day,9day,12day,15day convention calculate the number ofproliferating cells, detected the expression of CIK cell surface CD3,CD56by flowcytometry in the15day, the TLR1-TLR10mRNA expression of PBMC and CIK cells inthe9day,12day,15day were detected by RT-PCR method.Results1. Patients with nasopharyngeal carcinoma after CIK cell transfusion, compared with chemo-radiotherapy alone control group, the experimental group’s quality of life, physicalcondition and mental、psychological conditions improved significantly.2. PBMC stimulated by a variety of cytokines (INF-γ, IL-2, IL-1β, MabCD3etc.), thefirst6days begin to proliferate and reached a peak in about15days, flow cytometry cellsurface CD3, CD56show successfully cultured CIK cells.3. CIK cells compare with the control group peripheral blood mononuclear cells(PBMC), TLR1to TLR10expression differences: all the TLR receptors have differentlevels of expression in PBMC; in CIK cells TLR1expression enhanced, TLR4expressionweakened; TLR9in the two types of cells have higher expression.Conclusion1. CIK cells were able to improve the life quality, physical condition and mental stateof mind of patients with nasopharyngeal carcinoma, and no significant adverse reactions.Further proof that CIK cells adoptive immunotherapy to improve the quality of life, mentalstatus and immunity of cancer patients has unique advantages, may act as a tumor ofradiotherapy and chemotherapy adjuvant therapy program.2. Selective expression of TLR in PBMC and CIK cells，CIK cells kill tumor activitymay be related to the TLR expression.