The Main Composition Analysis Of Sambucas Williansii Hance And Sambucus Manshurica Kitag

Sambucas williansii Hance and Sambucus manshurica kitag belong to Caprifoliaceae、 Sambucus,is a kind of precious medicinal plants. The main function is broken bones、invigorate the circulation of blood、cure rheumatism bones and muscles pain. It grows the midwest of the province of Jilin and becomes the most important ornamental plant in China. Flavonoids is plant secondary metabolism prod and exists in plant leaves, roots, stems, flower and fruit. We have been found has more than5000kinds of flavonoids compounds in the food from now on. And the body can’t have flavonoids, we must have it from the food. So it became the hot spot and the frontier in the research. Sambucas williansii Hance and Sambucus manshurica kitag as raw materials to study, the result of the experimental as follow:(1) The paper research the base of the nutrition constituent、mineral element、the content of amino acid, the results show that the nutrition constituent of Sambucas williansii Hance and Sambucus manshurica kitag is rich and more comprehensive.(2) After a single factor experiment and the response surface method analysis, determine the best extraction process for Sambucas williansii Hance and Sambucus manshurica kitag.The best condition to ultra the total flavonoids from Sambucus mandchurica Hance were:ethanol concentration69.40%, ultrasound time58.04min and liquid solid ratio19.39mL/g, the extraction yield of the total flavonoids was4.0233mg/g. The three factors to influence the amount of flavonoid extract from big to small was:ultrasound time> liquid solid ratio> ethanol concentration.The maximum influence was ultrasound time. The best condition to ultra the total flavonoids from Sambucus mandchurica Hance were:ethanol concentration76.03%, ultrasound time48.86min and liquid solid ratio19.53mL/g, the extraction yield of the total flavonoids was5.79692mg/g. The three factors to influence the amount of flavonoid extract from big to small was:ethanol concentration>liquid solid ratio>ultrasound time. The maximum influence was ethanol concentration. (3) Through the state adsorption and the desorption contrast experiment, from eight kinds of macroporous adsorption resin. The best purification ability of flavonoid extract from Sambucas williansii Hance and Sambucus manshurica kitag was D101type. The best condition to purify the total flavonoid through the D101type from Sambucas williansii Hance was:the eluant is70%alcohol, current velocity of2mL/min, concentration of flavonoids of sample is5mg/mL, pH value is3,the degree of purify is18.9621mg/g More than4.0233mg/g of the before is improved by4.7times. The best condition to purify the total flavonoid through the D101type from Sambucas williansii kitag was:the eluant is70%alcohol, current velocity of2mL/min, concentration of flavonoids of sample is3mg/mL, pH value is3, the degree of purity is22.4746mg/g More than5.7969mg/g of the before is improved by3.8times.(4) The Sambucas williansii Hance and Sambucus manshurica kitag was ability to remove DPPH free radicals and superoxide radical, moreover, after purified by D101type, the ability obviously increased. The total flavonoids may be the main active component of Sambucas williansii Hance and Sambucus manshurica kitag which exerting an antioxidative activity.(5) After color response and high performance liquid chromatography (HPLC) the detection and control product comparison, in the same condition, the retention time of the rutin of standard substance was8.286min, the retention time of the unkown substance was8.325min.In the same time to have the peak. We can conclude preliminary the unkown substance was the rutin.