Establishment Of Controllable Spinal Cord Blast Injury Model And Related Research

The new features of modern war are the traditional bullets penetrating injuriesobviously are reduced but the number cases of shock wave damage are increasedby the bomb exploded. Although the main causes of death after injury areconcentrated in the chest, abdomen, brain and other organ damage, the survivingwounded, a large number of spinal cord injuries due to the shock wave.This subject is on the basis of preliminary studies, focused on the shock waveoverpressure range in causing the injury of the spinal cord surface, on the basis ofa relevant animal model to further observe the histological changes in the spinalcord blast injuries.Objective:To establish a new experimental animal model of shock wave damage, theshock wave direct role in the spinal cord surface, result in spinal cord injury,remove the interference of the skin, soft tissue, bones to the shock wave. Studythe physiological changes of the spinal cord after the shock wave damage andprovide a theoretical basis for the early treatment.Method: 1.10g of elemental manganese explosives (RDX) were used for underwaterexplosion source, electronically controlled detonation, measuring the intensity ofthe shock waves of different distances;2. Choose18rats and divided them into three groups,6rats per group. Thenremove the rats’ T9-10segment laminectomy and expose the spinal cord. Theexposed spinal cord is faceed to the direction of conduction of the shock wave, inaccordance with the distance from the explosion source0.3,0.8,1.5mimplementation of underwater explosions, detect transient overpressure peakexplosive distance. Observe the shock wave of positive duration of action, in24hours after spinal cord injury in rats survival and tissue injury, estimate the injurysituation.Results:1.After the explosion shock wave resulting in the death of experimentalanimals mainly due to: an important organ damage caused by respiratory andcirculatory failure. The primary reason is caused by pulmonary contusion andrespiratory failure;2.The explosion source as10g of elemental manganese explosives,3Mpaintensity of the shock wave1.5m distance from explosion source, after injury inexperimental animals completely survived, damage below the emergence ofmovement, sensory dysfunction, spinal cord damage score with the experimentalrequirements;24times were observed.3. After injury, the damaged spinal cord tissue can be seen a large number ofscattered hemorrhagic necrosis, hemorrhage necrosis of the gray matter of thescope of the heavier, significantly reduced the number of neurons within the graymatter, white matter demyelination like change.24hours after injury TEMobservation shows that neurons of the nucleus volume is reduced, within the fold formation, severe swelling of mitochondria, endoplasmic reticulum and otherorganelles, significantly reduced the number; myelin lamellar fracture anddisintegration of axoplasm within organelles reduced neurofilament fracture; graymatter found in a large number of red blood cells, accompanied by the formationof obvious necrosis,hemorrhage necrosis of the gray matter of the scope of theheavier, significantly reduced the number of neurons within the gray matter,white matter demyelination like change.24hours after injury TEM observationshows that neurons of the nucleus volume is reduced, within the fold formation,severe swelling of mitochondria, endoplasmic reticulum and other organelles,significantly reduced the number; myelin lamellar fracture and disintegration ofaxoplasm within organelles reduced neurofilament fracture; gray matter found ina large number of red blood cells, accompanied by the formation of obviousnecrosis.Conclusion: The accuracy and stability of the underwater controllability ofthe spinal cord blast injury model, the model excluding the skin, soft tissue, boneinterference, so that shock waves act directly on the spinal cord surface, a simpleshock wave factors injury characteristics for the the experiment provides a goodanimal model in the future.