Effect Of Nf-κB On Autophagy Formation Of Human Hepatoma Cell

Effect of NF-κB on autophagy formation of Human Hepatoma CellObjective:To research the effect of NF-κB on autophagy formation of Human Hepatoma Cell HepG2.Methods:The human hepatoma cell HepG2was cultured in vitro and divided into SN50group and control group, MTT assay was used to test the cell growth dynamically. Western blot analysis was performed to assess the expression of LC3-Ⅱ. The autophagy was observed usingfluorescent microscope by monodansylcadaverin (MDC) staining.Results:The inhibition ratio of HepG2cells were (20.45±3.1)%,(31.94±4.2)%and (36.44±4.6)%after treatment of SN50for24h,48h and72h, the difference was statistically significant than before (P<0.05). The expression of LC3-Ⅱ was upregulation than control group, and fluorescent staining reveal the activity of autophagy. The autophagy infected MDC fluorescent bulbs dark green or yellow-green punctate structures was observed under fluorescence microscope, most of them dispersed in the nucleus, indicating the After SN50role, hepatocellular carcinoma cells enhanced autophagy activity.Conclusion:Blocking of NF-κB can significantly inhibit the proliferation of HepG2cells in a certain period of time, and the effect may associated with increased cell autophagy.