Expressions And Significance Of CX43、CX40and C-JUN In Myocardium Of Patients With Sudden Cardiac Death

Object To detect the expression and signification of CX43、CX40and proto-oncogene proterns (C-JUN) in patients of sudden cardiac death.Method Forty SCD cases were chosen as the experimental group while ten cases of non SCD cases as the control group. Immunohistochemical staining was employed to examine the expression of CX43、CX40、C-JUN in myocardium. The SPSS18.0was used to analysis the Immunohistochemical results, and p<0.05as a statistically standards.Results1HE stainingIn the SCD group, most of them were died of coronary artery disease. And the myocardial slices of22cases had myocardial fiber fracture, myocardial interstitial edema, myocardial interstitial had a small amount of hemorrhage and lymphocytic infiltration, part of myocardial cells was hypertrophy and had fatty infiltration, eosinophilia was enhanced, part of myocardial nucleus was slightly increased, and abnormal nucleus was observed, chromosome was loose, part of myocardial cell was degenerate and necrotic, myocardial cells were slightly edema, fibrillar connective tissue increased in the myocardial interstitial. Left anterior descending coronary artery, circumflex branch and right coronary artery were noticed thickening in intimal the degree of luminal stenosis was>50%> part of the lumen occluded, part of lumen was thrombosed.2. Immunohistochemical staining①In control group, CX43mainly distributed on the end-to-end junction which were vertical to the long axis of the myocardial fiber,(intercalated discs).And a small part of the CX43distributed on the side-to-side junction which parallel to the long axis of the myocardial fiber. And the protein expressed on the intercalate discs as dense brown-yellow particles.But in the experiment group,CX43distributed as dispersion brown-yellow particles on the end to end and side to side junction.And there were disperse brown-yellow particles in the cytoplasm. The absorbance in the experiment group was higher than the control group (p<0.01).The expression of the CX40is lower than the CX43in ventricular myocardium. But in the atria myocardium the expression of the CX40was higher than CX43.But the distribution model was similar to the CX43.②The positive expression of C-JUN was detected in the cytoplasm in each case of SCD group. The C-JUN distribute in the cytoplasm, Tan particles was in the positive cells, showing point like, diffuse, dyeing evenly, The nuclek cell membranes and interstitial had no obvious dyeing, the expression of C-JUN in the group of SCD was significantly higher than in the negative control group (q=4.8150-8.8832, P<0.01)③The expression of CX43、CX40and C-JUN in myocardium of patients with SCD was negatively correlated(r1=0.315, r,=0.323, P,=0.022, P2=0.024)。 Conclusion1. The expression of the CX43and CX40were abnormal in myocardium.2. The remodeling of the CX43/40in myocardium induced the occurrence of the arrhythmia.3. The abnormal expression and the mechanism of the CX43/40in myocardium may be helpful to the drug research and development.Also it can be useful to the observation of the efficacy.4The expression of C-JUN was increased significantly in acute myocardium ischemia early in the patients of SCD, it is suggested that C-JUN may be involved in the pathogenesis of SCD. It may be become the pathology morphology auxiliary indexes to diagnosis the sudden cardiac death early, fast and sensitively.