Expression Of Leptin In Process Of Hepatic Fibrosis And Branched Chain Amino Acid On Liver Fibrosis Author

Objective: to observe the leptin on hepatic fibrosis and expression of TGF-beta1to explore the effect of branched-chain amino acid on the expression of both, study of branched-chain amino acids and the relationship between hepatic fibrosis and development, to provide a theoretical basis for the diagnosis and treatment of liver fibrosis.Method:random36Wistar rats are divided into3groups:normal control groups, model groups, and branched-chain amino acids in the treatment of hepatic fibrosis group. Hybrid modeling method of liver fibrosis model, introduced in the7th week of the branched-chain amino acids in the treatment group intragastric needle branched-chain amino acids in rats [(1.2g/(kg·d)], test completed in10weeks. Both HE and Masson staining for observation on histopathological changes in liver, while application of immunohistochemical detection of leptin, transforming growth factor beta1(TGF-beta1) expression in liver tissue and location, and using an image analysis system on both of positive results of correlation analysis, comparisons between groups of changes.Result:the composite model of successful completion of liver fibrosis model by copying. In made die subsection6week Shi, visible in rosette within and rosette between are has inflammation cell of invasion, in central vein wall and meeting districts are has comparison more of into fiber cell of appears; in8th weeks postmenstrual age model group in the appears liver cell of arranged disorder, liver cell of necrosis comparison heavy, performance for tablets shaped necrosis of while also with comparison more of inflammatory sexual cell of invasion, organization Zhijian and no connection; in10th weeks postmenstrual age model group of meeting districts has expanded, collagen fiber of hyperplasia more clear. Fibrosis model of the8th week and at10weeks, two sets of collagen of the percentage of area and normal control group when compared the difference statistically significant (p<0.01). After intervention of the branched-chain amino acids in the8th week and10weeks, branched-chain amino acids in the treatment groups can be seen infiltration of inflammatory cells and fibrous tissue hyperplasia and model compared with the same period of remarkably decrease the area as a percentage of their collagen and fibres in the same period of comparison of model groups is statistically significant (p<0.01). As model copy time of extended, liver organization of thin pigment, and TGF-beta1of expression are has different degree of increased, model group and normal of control group comparison has statistics of significance (p<0.01), model group Zhijian comparison also has statistics of significance (p<0.05); by branched-chain amino acids intervention Hou, treatment group8week and10week Shi of thin pigment, and TGF-beta1expression are than earlier of model group reduced (p<0.05). Expression of leptin expression is associated with the TGF-beta1(r=0.943,p<0.001).Conclusions as in made die process in the liver fiber of of occurs, and development, thin pigment and the TGF-beta1in liver organization in the of expression gradually enhanced, indicates that thin pigment and the TGF-beta1are participation has liver fiber of of occurs, and development; by branched-chain amino acids intervention Hou, thin pigment and the TGF-beta1of expression are different degree of reduced, large rat of liver fiber of degree also gradually reduce. Therefore, the branched-chain amino acids as a therapeutic target for liver fibrosis, may more effectively inhibit liver fibrosis development.