The Expression Of Runx3、TGF-β1、β-catenin And Cyclin D1in Primary Liver Cancer And Their Correlation Studies

Objecrive The subject is to detect the expression of Runx3、TGF-β1、β-catenin and CyclinD1in Liver tissues and their corresponding adjacenttissues and normal liver tissues，and to analyze their correlations betweenthem.To have a preliminary study of them in PHC occurrence、development、invasion、metastasis etc，and to understand the interactions between theirrole of signaling pathways.Mehods Two-step non-biotin immunohistochemical method was performed toseparately detect the expression of Runx3、TGF-β1、β-catenin and CyclinD1protein in45PHC tissues and their corresponding adjacent tissues and45normal liver tissues.Results (1) The positive expression rate of Runx3in Liver tissues was35.6%(16/45),the expression rate was significantly lower than thecorresponding adjacent tissues60%(27/45) and normal livertissues80%(36/45),the difference between then was significant(P all＜0.05).The abnormal expression of Runx3was associated with portal vein tumorthrombus,clinical stage, capsule and lymph node metatasis(P＜0.05),and hadno correlations with gender、age、AFP、HBsAg、histological grade and tumorsize(P＞0.05).(2) The positive expression rate of TGF-β1in Liver tissueswas40%(18/45),the expression rate was higher than the correspondingadjacent tissues20%(9/45) and normal liver tissues4.4%(2/45),the difference between then was significant(P all＜0.05).The abnormal expression of TGF-β1was associated with histological types、portal vein tumor thrombus、lymph node metatasis、capsule、AFP and tumor size(P＜0.05),and had nocorrelations with gender、age、clinical stage and HBsAg (P＞0.05).(3) Thepositive expression rate of β-catenin in Liver tissues was51.1%(23/45),theexpression rate was higher than the corresponding adjacenttissues26.7%(12/45) and normal liver tissues8.9%(4/45),the differencebetween then was significant(P all＜0.05).The abnormal expression of β-catenin was associated with portal vein tumor thrombus、capsule、tumor stage、intrahepatic or distant lymph node metatasis and HBsAg(P＜0.05),and had norelationship with gender、age、tumor diameter、 histological types and AFP(P＞0.05).(4) The positive expression rate of CyclinD1in Liver tissues was87.5%(21/45),the expression rate was significantly higer than thecorresponding adjacent tissues24.4%(11/45) and normal livertissues6.7%(3/45),the difference between then was significant(P all＜0.05).The abnormal expression of CyclinD1was associated with portal veintumor thrombus、clinical stage、histological type、lymph node metatasis andHBsAg(P＜0.05),and had also no relationships with gender、age、tumor size、capsule and AFP(P＞0.05).(5) The expression of Runx3、TGF-β1、β-cateninand CyclinD1were all associated with portal vein tumor thrombus and lymphnode metatasis(P＜0.05).(6) The protein expression between any two ofRunx3、TGF-β1、β-catenin and CyclinD1were related:Runx3and TGF-β1：X~2=23.341, P=0.000；Runx3and β-catenin：X~2=10.405, P=0.001；Runx3andCyclinD1：X~2=7.774, P=0.005；TGF-β1and β-catenin：X~2=10.020, P=0.002；TGF-β1and CyclinD1：X~2=16.205, P=0.000；β-catenin and CyclinD1：X~2=14.032, P=0.000，(Pall＜0.05).Conclusions The expression correlation of Runx3、TGF-β1、β-cateninand Cyclin D1implied that：their role of different signaling pathways existinteractions,may co-involved in the occurrence of PHC、 development、progression and metastasis.To have a combined detection of their expressionin PHC can contribute to the early diagnosis and prognosis of PHC,and mayprovide gene therapeutic targets for the treatment of PHC molecular biology.