Zinc Finger Protein ZBTB20Effect And Mechanism In The Memory

The mammalian hippocampus harbors neural circuitry that is crucial for associativelearning and memory. Its mechanisms are not yet fully understood. Our previous studyshowed that ZBTB20played a key role in the differentiation and development of thehippocampus. Whole body ZBTB20gene knockout lead to a serious obstacle tohippocampal development in hippocampal CA1neurons in the molecular phenotype, cellmigration and cell body arranged adjacent transitional cortex features, increase in apoptosisof hippocampal neurons and hippocampal nerve projection exception. Therefore, we usedgene targeting technology to build the model of the ZBTB20hippocampal CA1region-specific knockout (CA1-ZB20KO) mice. Observed in the the ZBTB20hippocampalCA1region-specific knock in addition to the basic structure of the pyramidal cells in mice,morphology, activity, synaptic transmission function, no obvious change. However, we showthat conditionally deleting Zbtb20specifically in mature CA1pyramidal neurons impairedhippocampus-dependent memory formation, without affecting hippocampal architecture orthe survival, identity, and basal excitatory synaptic activity of CA1pyramidal neurons. Wedemonstrate that mature CA1-specific Zbtb20knockout mice exhibited reductions inlong-term potentiation (LTP) and NMDA receptor (NMDAR)-mediated excitatorypost-synaptic currents. Furthermore, we show that activity-induced CREB phosphorylationis impaired in the hippocampal CA1of Zbtb20mutant mice. Collectively, these resultsindicate that Zbtb20in mature CA1plays a critical role in LTP and memory by regulatingNMDAR activity and CREB activation.