The Effects Of ISL-β-CD Complex On The RA Induced By Type Ⅱ Collagen In Rats

Objectives To study the effects of ISL-β-CD for rheumatoid arthritis （RA） induced bysheep type Ⅱ collagen protein. To provide a new therapy for RA and a newproof for furtherresearch.Methods We prepared the mixture of sheep typeⅡ collagen protein andCFA first. Thenwe got100SD rat, and chose ten from them random as blank control group. The others wereinjected with the above mixture intradermally at the right hindfoot, and injected again oneweek later to booster. Then we chose the qualified ones and separated them into five groupsand ten rats each, as model group, glucoside tripterygium group(100mg·kg^-1)and threeISL-β-CD groups with different doses(100mg·kg^-1，400mg·kg^-1，800mg·kg^-1). All rats wereadministrated by oral once a day for four weeks. During the period, we weighted them andmesured the volume of the right hindfoot once a week. After four weeks, we anesthetized allthe rats, collected blood and draw the synovial tissue of the joint. We measured the levels ofSOD、MDA、IL^-1β、TNF-α、IL-6、IL-4and IL^-10in serum by ELISA, counted the apoptosedcells in synovial tissue by TUNEL, and detected the express of Cyclin D1in synovial tissueby western-blotting.Results1. In ISL-β-CD groups, the rats weight significantly increased, the joint swelldegree and arthritis score decreased obviously（P＜0.05）. ISL-β-CD can increase the weight of CIA rats, decrease the degree of joint swelling.2. In ISL-β-CD groups, the level of SOD inserum increased and the level of MDA in serum decreased （P＜0.05）. ISL-β-CD can increasethe level of SOD and reduse the level of MDA in serum.3. In ISL-β-CD groups, the level ofIL-4,IL^-10in serum increased and the level of IL^-1β, TNF-α and IL-6in serum decreased （P＜0.05）. ISL-β-CD can increase the level of anti-inflammatory cytokines, reduse the level ofpro-inflammatory cytokines in serum.4. In ISL-β-CD groups, the pathologic change ofsynovium of joint improved. ISL-β-CD can improve the the pathologic change of synoviumof joint.5. In blank group, there were few cells in apoptosis, the same with that in modelgroup. And that in ISL-β-CD groups are more than the above groups（P＜0.05）. ISL-β-CD caninhibit the apoptosis of synovial cells.6. In ISL-β-CD groups, the expressions of Cyclin D1in synovial tissue were the same with that in model group（P＞0.05）. ISL-β-CD cannot reducethe expression of Cyclin D1in synovial tissue.Conclusions1. ISL-β-CD can reduce the inflammatory reaction of CIA rats, improvethe the pathologic change of synovium of joint. There is a therapeutic action on CIA rats.2. ISL-β-CD cannot reduce the expression of Cyclin D1in synovial tissue, so we guessISL-β-CD restrain cell hyperplasia by other factors.