| Background Herpes simplex virus encephalitis (HSE) is the most common acute focalvirus encephalitis among adults. Most patients have a series of cognitive dysfunctionperformance. Cyclin-dependent protein kinase5(CDK5) has been confirmed to be related tocognitive function.In this study, we investigated the change of the expression and possibleeffect of CDK5in hippocampal tissue of mice with HSE associated cognitive outcome.Methods A total of one hundred male C57BL/6mice were randomly assigned to thefollowing three groups: the normal group,the sham-operated group and the model group. HSEmouse model was established by intracerebral inoculation. The Morris Water Maze test wasperformed to investigate the changes in the spatial memory and cognitive function of eachgroup20days after inoculation. Semiquantitative RT-PCR was used to measure CDK5mRNA level.Western blot analysis was used to evaluate the protein levels of CDK5and itsactivator p35/p25.Results Compared with the sham-operated group and the normal group, the modelgroup exhibited impaired spatial learning and memory(P<0.05). The level of CDK5mRNA,as well as CDK5and p35/p25protein levels in the model group were significantly higher thanthat of the sham-operated group and the normal group (P<0.05).Conclusion Mice with HSE displayed obvious cognitive impairment.CDK5may belinked to the the development of cognitive outcome of mice with HSE.Key words Herpessimplex virus encephalitis; Cognitive deficits; CDK5; p35/p25... |
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