Effct Of Fructose1,6-Diphosphate To HIF-1α And VEGF On Ischemic Reperfusion Of Cardiac Muscle In Rats

Objective: To investigate the serum of cTn1, HIF-1α and VEGF inischemia-reperfusion rat after intervention of FDP. To investigate the possibleprotective mechanism of myocardial hypoxic-ischemic injury.Method:56SD rats were randomly divided into3groups: group SH (shamoperation group, were divided into4subgroup,2per group); group I/R(ischemia-reperfusion group, were divided into4subgroup,6per subgroup);group FPD (intervention group, were divided into4subgroup,6per subgroup)were injected FDP1g/kg. Two rats were sacrificed at the time of6h,12h,24h, and72h after injection in group SH.6rats were sacrificed at the time of6h,12h,24hand72h in the modeling in other groups. To observe the rat of death in every group;Compare the cTn1, HIF-1αand VEGF among the each groups, collect themyocardial samples, observe the change of tissue in myocardial..Results1. The number of deaths in each group: group SH were no death,5rats died ingroup I/R;2rats died in group FDP.2. cTn1: There were no significant different in group SH at the each time point;the value of cTn1raised up at6h, highest at24h and decreased at72h in groupI/R. There were significant difference between group SH and I/R(P<0.01).There were significant difference between group FDP and SH (P<0.01). Therewere significant difference between group FDP and I/R(P<0.01).3. HIF-1α: There were no significant different in group SH at the each time point; value of HIF-1α was highest at6-12h and decreased at24h. There weresignificant difference between group SH and I/R (P<0.01). There were nosignificant difference between group SH and I/R at72h; There were significantdifference between group FDP and SH except at72h (P<0.05); There weresignificant difference between group FDP and I/R (P<0.05).4. VEGF: There were no significant different in group SH at the each time point;the level of VEGF was highest at6-12h and decreased at24h. There weresignificant difference between group SH and I/R.(P<0.01). There were nosignificant difference between group SH and I/R at72h; There were significantdifference between group FDP and SH except at72h (P<0.05); There weresignificant difference between group FDP and I/R (P<0.05).5. Myocardial histopathology: group SHrat myocardial cells arranged in neatrows and close, no inter-organizational edema, a small amount ofinflammatory cell infiltration; group I/R myocardial cell derangementirregular, edema, inflammatory cell infiltration, visible necrosis District;group FDP myocardial cell were found mild edema, inflammatory cellinfiltration decreased, without necrosis.Conclusions1.. FDP has a protective role on ischemic and reperfusion myocardial injury.2. When myocardial was injury after ischemia-reperfusion, the serum of HIF-1αand VEGF were significantly increased, which suggested that HIF-1α andVEGF were played a role in the pathological changes of myocardial ischemiaand hypoxia.3. FDP pretreatment can reduce the cardiac enzymes, and reduced the myocardialdamage from ischemia; which may association with reduced the HIF-1α andVEGF level in serum.