Experimental Study Of Effect Of Celastrol And Triptolide On Prostate Cancer Cell

Prostate cancer is a major problem which affects men’s health, and more than 220,000 deaths are due to the disease in worldwide annually. Although previous study has made great progress in diagnosing and treating of prostate cancer, but how to remove and cure prostate cancer completely is still a mystery.In our study, we adopted the small molecular compound celastrol and triptolide to treat androgen-independent PC-3 cells and androgen-dependent LNCaP cells, then observed the effects of medicine on growth, proliferation, apoptosis of PCa cells, and detected the change of prostate-specific antigen level. We proved that the two medicine can inhibit the growth and proliferation of PCa cells, that provided the cytological basis for the follow-up drug anti-cancer research.In our study, we adopted 0.1μM, 1μM and 10μM celastrol and triptolide to treat PC-3 and LNCaP, then used trypan blue staining, counted cell numbers and drew growth curve to detect the effect of medicine on growth of PCa cells. The analysis of half cells inhibition rate IC₅₀ was conducted by MTT method. We double dyed cells and detected the apoptosis by fluorescence microscope and flow cytometer. PSA level was measured by chemiluminescence method. The results indicate:（1） 0.1μM celastrol can inhibit growth of PCa cells in a certain extent, but 0.1μM triptolide completely inhibited growth of PCa cells, thus the inhibit effect of triptolide was better. The growing speed of PCa cells treated by 0.1μM celastrol was more slow than control cells’. 1μM Celastrol had completely inhibited the growth of PC-3 cells, and 10μM celastrol can make PC-3 cells die; 1μM Celastrol slowly inhibited the growth of LNCaP cells, and the whole of cells were dead when celastrol treated them five days. The whole of LNCaP cells were dead when they were treated with 10μM celastrol for 72 hours. However, there is no difference in the inhibiting effect of the three concentration 0.1μM, 1μM, 10μM triptolide on PCa cells.（2） IC₅₀ of PC-3 and LNCaP cells treated by celastrol is respectively 1.16μM, 2.57μM, and IC₅₀ of PC-3 and LNCaP cells treated by triptolide is respectively 274.60nM, 149.45nM. 0.1μM Celastrol had inhibited the proliferation of PC-3.Starting from 0.5μM celastrol, the inhibiting effect of celastrol on PC-3 cells was more obvious following concentration increasing; The inhibiting effect of 0.1μM celastrol on LNCaP cells wasn’t obvious, from 0.5μM starting, the inhibiting effect was more obvious following concentration increasing. However, the inhibiting effect of 0.005μM triptolide on the two PCa cells was very obvious, and the inhibiting effect was more obvious following concentration increasing. Therefore, the two medicine can inhibit growth and proliferation of PCa cells, and the inhibiting effect of triptolide is more obvious.（3） The two medicine can induce cells apoptosis, and that triptolide induces cell apootosis is more obvious. The appearance that LNCaP cells are induced apoptosis is more clear than PC-3 cells apoptosis.（4） These medicine can reduce PSA level, and triptolide reduces the PSA level more significantly, in addition, the drop extent of PSA level that medicine treat cells for 48 hours is more obvious than 24 hours’.Therefore, we find that celastrol and triptolide can inhibit growth, proliferation of PCa cells, induce apoptosis and reduce PSA level. All kinds of effects of triptolide on prostate cancer cells are more obvious than celastrol.