Study On Fibrinolytic Compound MTP Enhancing Fibrinolysis In Rats

The effects of compound MTP on clotlysis induced and its possible mechanism were investigated with compound MTP as a promoting fibrinolytic compounds injected in the tail vein of rats and pulmonary thrombotic model on rats established by marking fibrin with FITC. SDS-PAGE,fluorescence value of plasma and lung tissue slices in various groups were assayed. The present results provided first evidence that compound MTP led to enhanced fibrinolysis in vivo. It was speculated that compound MTP could enhance intrinsic activity of prourokinase as a plasminogen activator in a rat pulmonary embolism model.First of all, pulmonary thrombotic model on rats was established by marking fibrin with FITC. Through the lung tissue slices to estimate whether the FITC- pulmonary thrombotic model on rats was successful. Compared pulmonary thromboembolism in the control group slices with the control group, it was found that the pulmonary thrombosis had formed in the Rat pulmonary embolism model.Then, pulmonary thrombotic model on rats was established by marking fibrin with FITC. The single-chain urokinase-type plasminogen activator group and saline group were divided as the positive control group, control group respectively.compared with the low MTP- dose treatment group, middle MTP- dose treatment group and high MTP- dose treatment group. At 20min, 1h, 2h, 3h, 12h, 24h, 36h , SDS-PAGE ,fluorescence value of plasma and lung tissue slices in various groups were assayed. The fibrinolysis activities of MTP high, medium and low dose were evaluated.Through the lung tissue slices,it was found that the alveolar wall of low MTP- dose treatment group, middle MTP- dose treatment group and high MTP- dose treatment group had thickened,but,there was no thrombosis in capillary. It was speculated that the formed thrombus had been dissolved under the effect of MTP. But there was still tissue damage that caused by pulmonary thromboembolism.The degradation state of FITC-fibrin was assayed. It was found that the fluorescence intensity of the plasma producing by degradation of FITC-thrombosis increased rapidly after MTP was injected into the tail vein of rats. In FITC-pulmonary thrombotic model, the fibrinolytic activity of blood increases after MTP was injected into the tail vein of rats.It caused the highest fluorescence intensity at 2h.The fluorescence intensity of degradation producted FDP3 and FDP4 form high-dose treatment group was close to the fluorescence intensity of degradation product FDP3 and FDP4 form positive control group. The molecular weight of FDP3 and FDP4 was about 100kDa and 50kD, they were tantamount to the molecular weight of fragments D(100kD) and fragments E(50kD) about fibrin degradation products. Therefore, fragment D and fragment E of fibrin degradation products could be used as a marker of thrombolysis.The fluorescence value of plasma about positive control group, control group, low MTP- dose treatment group, middle MTP- dose treatment group and high MTP- dose treatment group were assayed.It was found that adding the MTP of 5mg/kg promote the dissolution of thrombus. This result was similar to the positive control group.After SDS-PAGE,fluorescence value of plasma and lung tissue slices in various groups were assayed and we concluded that MTP had effect on thrombus dissolution as a fibrinolytic activity compound from the marine microbial. The terpenoid compound MTP had the excellent effect of thrombolysis and broad application prospects.