The Study Of Anti-experimental Liver Injury Action And Mechanism Of Salvianolic Acid B

ObjectiveSalvianolic acid B(SA-B) is a main ingredient of traditional Chinese herb-Dansheng(Salvia miltiorrhiza Bge.). To study the anti-experimental liver injury action and mechanism of SA-B, We set up the animal model of chemical Liver Injury induced by Dimethylnitrosamine(DMN) and Immune Liver Injury induced by Concanavalin A to evaluate the therapeutic effect, and to provide the pharmacology proof of SA-B that be utilized in clinical treatment. Experimental contents 1.Protective Effects of SA-B on Hepatic Injury in Mice Induced by DMNSixty male NIH mice were randomly divided into five groups:normal control group(group A), model group(group B), high dosage group of SA-B (group C), low dosage group of SA-B (group D), and bifendate group (group E). Excepts those in group A, mice in other groups were injected with dmn (dose of 15mg/kg) via the intraperitoneal at the first day of experiment. Group C and group D were orally administered with SA-B at does of 160mg/kg and 80mg/kg respectively. Group E were orally administered with bifendate at the dose of 150mg/kg. All the drugs were given every 12h. At the 48h, Blood sample for determineing aminotransferase (ALT) and TNF-a levels were collected, Histopathological examination was performed for liver tissue.It showed that the serum levels of ALT and TNF-a in both groups of SA-B were obviously lower to that of group B(P<0.01), Serum levels of ALT and TNF-a in group C were obviously lower to that of group D (P<0.01). but have no significant difference comparing with that of group E(P>0.05). hepatic histopathological change of necrosis were alleviated in both groups of SA-B. It suggested that SA-B possess the effects of protecting liver from chemical injury in mice induced by DMN. 2. Protective Effects of SA-B on immune Hepatic Injury in Mice Induced by Concanavalin ASixty male NIH mice were randomly divided into five groups:normal control group(group A), model group(group B), high dosage group of SA-B (group C), low dosage group of SA-B (group D), and bifendate group (group E). Excepts those in group A, mice in other groups were injected with Con A (dose of 20mg/kg) via the tail vein at the first day of experiment. Group C and Group D were orally administered with SA-B at does dose of at does of 160mg/kg and 80mg/kg respectively. Group E were orally administered with bifendate at the dose of 150mg/kg. All the drugs were given once daily for 5 days consecutively. At the last time, when drugs were administered for 2 hours, mice were injected with Con A once again at the same dosage. Blood sample for determineing aminotransferase (ALT) and IL-6 levels were collected at the 8th hour after Con A administration. Histopathological examination was performed for liver tissue, the expression of ICAM-1, Fas and FasL were detected by immunohisttochemical method.It showed that the serum levels of ALT and IL-6 in both groups of SA-B were obviously lower to that of group B(P<0.01), Serum levels of ALT and IL-6 in group C were obviously lower to that of group D (P<0.01), but have no significant difference comparing with that of group E(P>0.05). hepatic histopathological change of necrosis were alleviated in both groups of SA-B. the experssion of ICAM-1, Fas and FasL in liver tissue of group C and group D were obviously attenuated comparing with that of group B(P<0. OK 0.05). It suggested that SA-B possess the effects of protecting liver from immune injury in mice induced by Concanavalin A. Conclusion1. SA-B possess the effects of protecting liver from chemical injury in mice induced by DMN by suppressing the production of TNF-a.2. SA-B has a good effects of protecting liver from immune injury in mice by inhibiting the secretion of IL-6, and by suppressing the expression of ICAM-1, Fas and FasL.