Neuroprotective Effects Of Epigallocatechin-3-gallate On Primary Hippocampal Neurons Of SAMP8 Mice

Alzheimer’s disease is a primary neurodegenerative brain disease,the main pathological features include three aspects: the formation of senile plaques, neuronal degeneration or loss and neurofibrillary tangles. EGCG is the main component of tea polyphenols in the body in a wide range of biological activities, which will help prevent diseases associated with oxidative stress, such as Alzheimer’s disease, Parkinson’s psychosis. With the advent of global aging and the increasing pressure of social competition, AD increasing the incidence of, and has affected the quality of life of patients and their families, so the development of drug treatment of AD is imminent. [Objective] EGCG in mice for SAMP8 hippocampal neurons during aging in rats. [Method] extracting one new SAMP8, SAMR1 mouse primary hippocampal neurons. Hippocampal neurons will be seeded in 35mm diameter Petri dishes in culture medium containing 20% fetal bovine serum to DMEM, 24h after the replacement of the culture medium was Neurobasal +2% B27, culture medium was changed again after 72h, cell culture to 4 days Different concentrations of EGCG, were cultured for 13 days. 1. Determined appropriate by MTT EGCG concentration; 2. immunohistochemistry of EGCG observed protective effect on neurons; 3. with hybridization method to detect tau protein phosphorylation pathway in cdk5, ptauS199,ptauS396 expression. [Results] 1.MTT results show that: treatment with different concentrations of EGCG SAMP8 mouse hippocampus and found: the concentration of> 20μg/mL of EGCG inhibited the growth of neurons and lead to his death, and the concentration of <20μg/mL time Protective effect on neurons is not obvious, the concentration of EGCG 20μg/mL could significantly promote the growth of neurons. 2. immunohistochemistry: the experimental group (SAMP8 +20μg / mLEGCG) after treatment with EGCG inhibited the SAMP8 mouse primary hippocampal neurons in cell aging, the results with the control group (SAMR1) levels consistent. 3.Western blot: EGCG treatment cdk5, 396,199 significantly decreased the phosphorylation level, close to the normal control group (SAMR1) level. [Conclusion] 1.SAMP8 mouse hippocampal neurons in culture showed the process of premature aging, this study, cell viability and morphology of the two come to the suitable concentration of EGCG can delay the appearance of aging. 2.EGCG the protective effect is reduced cdk5, ptau199,ptau396, three further increased Gsk3βreduced the phosphorylation level of neurofibrillary tangles to achieve.3.EGCG by increacing the expression of Bcl-2 levels by the appearance of apoptosis.