Genetic Diversity Of Salix Psammophila From Different Producing Areas Analyzed By SSR Markers

Salix psammophila is a profusion of unique deciduous shrub, is the dominant tree species in afforestation in sandy areas.In this study, 330 Salix psammophila in 8 areas were used to conduct genetic analysis by using SSR markers. Among 45 pairs of SSR primers, 19 pairs of primers were polymorphic. A total of 74 bands were amplified, in which 63 were polymorphic. The number of the allelic loci for each pair of primers was ranged from 2 to 11, and was 3.89 on average. The average of effective number of alleles(NE), expected heterozygosity(He), observed heterozygosity(Ho), Shannon’s information index(I) were 1.8171,0.4079,0.3798,0.6447. Based on the amplified SSR bands, genetic similarities among eight groups of Salix were varied from 0.9437 to 0.9908, using POPGENE. C luster analysis showed that the 8 groups are divided into 3 classes, according to the UPGMA method. The results showed that there was a positive correlation between the genetic diversity and geographical origin.Through eight different origin groups comparison, two groups’ Ne, He and I value a high level of genetic variation is more abundant, indicating that the two populations have the potential origin of Salix psammophila selective breeding in the future should be strengthened protection.