The Expression Analysis Of Four Genes Of Apostichopus Japonicus

Apostichopus japonicas belongs to Echinodermata, Eleutherozoa, Holothuroidea, Aspidochirota, Stichopodidae. It is normally restricted to the shallow sea of China, Korea and Japanese in the Western Pacific, and is liked by their people. Today, Apostichopus japonicas’ quality is uneven, we need to solve the fundamental problem in deeper level.This study we researched the expression of four genes of Apostichopus japonicas used real-time quantitative PCR to research the growth of A. Japonicus. we selected the four functional genes which were closely linked with the sea cucumber to verify its expression quantity relationship to grow. They were tenascin, collagen α2, integrin αⅤ and integrin βL. Then, we took eight tissues of 30 Apostichopus japonicas respectively for experiments, the 30 Apostichopus japonicas were be a group. We took a group of every other month, here is a total of three groups.The results showed that there were different degree expression levels of tenascin gene in intestines, tube foot, respiratory tree, skin, body wall, coelomic fluid, papilla and longitudinal muscle at three time points in A. japonicus. But the expression levels were higher in coelomic fluid and respiratory tree than in other tissues(P<0.05). The expression levels were increased of collagenα2 gene with month age increasing in tube foot, respiratory tree, body wall, papilla and longitudinal muscle. The expression level was the highest in respiratory tree, and then declined gradually in longitudinal muscle, papilla, body wall, intestines and coelomic fluid, respectively. The expression levels were gradually increased of integrin αV gene with month age increasing in longitudinal muscle, papilla and body wall, respectively. And the expression levels were lower of integrin βL gene in skin, body wall, papilla and tube foot. The results of this study provided foundation for further studying this four genes in A. japonicus.