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The Residual Organic Arsenic Additives In Liver Kidney And Effect On The Farms Surrounding Environment

Posted on:2017-02-08Degree:MasterType:Thesis
Country:ChinaCandidate:X J ChangFull Text:PDF
GTID:2283330503963722Subject:Public health
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[Objective]1. To investigate the Fenyang City farm environment medium arsenic exposure level and compared to the control group, understand the level of organic arsenic external environment pollution, namely the external exposure levels, estimation of arsenic in feed addition, inquiry manure, soil arsenic and arsenic in feed level correlation.2. Knowledge of organic arsenic feed additive of pig blood parameters influence and correlation of organic arsenic content with pig blood parameters change, from blood science angle of arsenic targets and mechanisms.3 In the liver and kidney to arsenic residues, and provides the basis for the actual animal source of arsenic in food residues.[Methods]Shanxi Province Fenyang City four pig field pigs as exposure group, pigs are to buy fattening feed, do not feed feed pigs served as controls. The acquisition of pig fattening feed, manure mixed, the Pig Manure Fertilization of soil on each sampling point both north and south, East and West four range of soil and mark- 20 DEG C preservation. Collect 5ml venous blood of pigs, do the blood routine test right now. Take the liver, kidney and muscle samples in slaughtering production line, back to the lab-20 ℃refrigerator. Feed, feces, arsenic content in soil by microwave digestion, and organ into meat, wet digestion, and then the hydride generation atomic fluorescence spectrometry determination of arsenic content.1. Preliminary assessment of arsenic pollution in ecological environment[Results]Four pig feed, soil, fecal arsenic concentrations were higher than the control group and total arsenic in feeds high concentration of pig farms, the fecal concentrations of arsenic also corresponding increase, long-term arsenic content in the Manure Fertilization of soil is high. The pig feed B and soil arsenic content have significant correlation, r=0.961.1Luocheng, ancient Fu Tu Cun, Dong Si Cun three village four small farm soil arsenic are exceeded, the mean content is 97.06,117.02,173.97, 53.64mg/kg. Compared with the control group, the soil arsenic concentration of high arsenic exposure group, the order of C>B>A>D, and the difference was statistically significant(P<0.05).1.2 Four pig farms breeding pig manure mixed with arsenic content is 36.14,80.52182.85,43.42mg/kg. Arsenic exposure group of soil arsenic concentrations were higher than those of the control group and four pig farms on the arsenic content in order C>B>D>A, greater than reference standard value of 30 mg / kg, and the difference was statistically significant(P < 0.05).1.3 Four pig feed arsenic content were is 16.6,93.08,125.5,30.92mg/kg.Compared with the control group, the arsenic concentration in the arsenic exposed group was higher, the order was C>B>D>A, and the difference was statistically significant(P<0.05).1.4Arsenic exposure group of feed, soil, fecal arsenic concentrations were higher than control group, and arsenic content in four pig farms in order of size are C>B>D>A, namely feed total arsenic high concentration piggery, dung arsenic concentration also corresponding increase, long-term with the Manure Fertilization of soil arsenic content in is also high. Correlation of feed and farm soil arsenic were done, including pigsty B feed and soil arsenic content have significant correlation, r = 0.96)(P < 0.05).2. Changes in peripheral blood parameters of pigsProcessing feed for pigs and non feed fed to pigs compared to blood parameters have changed. The content of leucocyte, erythrocyte mean corpuscular volume, average hemoglobin concentration, platelet count, red blood cell distribution width, platelet volume fraction results(P < 0.05), the difference is statistically significant; further bivariate correlation analysis showed that, mean corpuscular hemoglobin and red blood cell distribution width and feed arsenic content had significant correlation.3.arsenic residue in pig liver and kidneySwine a liver arsenic ranges between 0.108-0.509, pigsty B between 0.215-0.673. C between 0.447-1.066. D between 0.092-0.788. Control group between 0.004-0.094 exposure group, the liver arsenic content was significantly higher than that of the control group, and P < 0.05 have statistical significance. Some samples exceeded the standard higher than China’s regulations limit of 0.5 mg / kg, a, B, C, D farm exceeded rates were 11.1%, 16.7%, 41.7%, 5.6%, 0% in the control group, which C farm exceeded rate is higher, and average arsenic level is the highest.[Conclusions]1. Organic arsenic in feed may be added concentrations of arsenic in the environment caused by increased accordingly, including manure, Pig Manure Fertilization of soil arsenic levels are much higher than the background value or the reference control standard. The arsenic through soil, water entering the food chain enrichment by increase the concentration of arsenic, thereby affecting human health.2. Organic arsenic in feed addition may cause swine peripheral blood parameters change, suggesting that the target for the action of arsenic may is the blood system.3. Pigs liver and kidney were arsenic residues, including liver residues more high, arsenic residues exceeded the phenomenon, Fenyang City feed use, lax supervision, feed manufacturers have private indiscriminate, adding excess phenomenon, should arouse the attention of the relevant departments.
Keywords/Search Tags:Organic arsenic, ecological environment, pig peripheral blood
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