Effects Of Polymorphisms Of PDK1、FoxO3 And Akt3 Gene On Production Performances In Yorkshire

In this study, Sequenom and DNA sequencing technology were used to detect the polymorphisms of PDK1, Foxo3 and Akt3 gene which are associated with the growth traits of pig. In total 8 SNPs were detected, and the genetic structure, genetics effects of the SNPs on production performances were analyzed in a Yorkshire population. The results are as follows: 1.The Detection of Polymorphisms of PDK1 Gene and the Effects of the Polymorphisms on Production Performances3 SNPs of PDK1 gene were detected:T2262C,A6970C and G8149A, and the detected genotypes are CC,TT,TC;AA,CA;AA,AG,GG; respectively.Only T2262C was misssense mutation. To this site,both the corrected age of reaching 100kg (CA100) and the Backfat of TT were significantly higher than that of CT ((P<0.05, P<0.05)).2. The Detection of Polymorphisms of Foxo3 Gene and the Effects of the Polymorphisms on Production Performances3 SNPs of FoxO3 gene were detected:G82316A、T60086C and A64011G,and the detected genotypes are AA,AG,GG;TT,TC,CC;AA,AG,GG; respectively.To SNP G82316A, both the CA100 and the Backfat of AA are significantly higher than that of AG(P <0.05, P<0.05) and GG (P<0.05, P<0.05).To SNP T60086C, the CA100 and the Backfat of CC were higher than CT (P<0.05, P<0.05). The Corrected eye muscle area of reaching 100kg (CEMA100) of CC is significantly larger than CT (P <0.05) and TT (P<0.05).To 64011G, the Backfat of AG was significantly thinner than that of AG (P<0.05) and GG (P<0.05); the CA100 of AG was significantly lower than that of AG (P<0.05) and GG (P<0.05).3. The Detection of Polymorphisms of Akt3 Gene and the Effects of the Polymorphisms on Production Performances2 SNPs of Akt3 gene were detected:T206453G and A210280C, and the detected genotypes are TT,TG,GG and AA,AC,CC;respectively.To SNP T206453G, The CA100 of GG was significantly higher than that of TT (P<0.05). The BF of GG was significantly higher than that of TT (P<0.05).