Study On The Genetic Analysis Uncultivated Auricularia Heimuer Germplasm Resources And The Development Of Good Strains And New Matrix

Under the funds of the " Auricularia heimuer breeding station of national modern Auricularia heimuer industry technology system ", this paper used the wild Auricularia heimuer strains from different regions of Changbai Mountain and the Greater Xing’ an Mountains and different host species, compared with the strains from different or same Quercus acutissima’s wood blocks and places by natural inoculate in Yunnan Province. genetic analysis of the above-mentioned germplasm resources, the research of the biological characteristics, screening of good bacterial strains, and using the reed as a new matrix to study the law of nutrition utilization, agronomic traits, optimized the best cultivation formula Intended provide germplasm resources for genetics and breeding, in order to make the law of nutrition utilization clear and provide good bacterial strains and ancillary cultivation techniques for production.(1)To evaluated the results of somatic incompatibility and esterase are enzyme experiment the wild Auricularia heimuer show that the bacterial strain has more differences and distant genetic relationship between north and south are; There are differences between strains of the same area strains on different host species in Northeast China, on the same host species still possible; There are also differences between strains between different hosts on the same fallen wood specie and the same position on the same host strain of the southern region, but most of strains exactly similar, does not produce the phenomenon of antagonism, to determine the strain as the same species when the correlation coefficient reached 1.00. Measured migration rate with29 distinct enzymes bands, the band number between 9-18, distribution of mobility rate between Rf0.076-Rf0.987, which was 0.374 migration band E11 for 18 strains of the bands. Overall, the growth of the 18 kind of strains was different hosts, regions, habitats, even the growth situation of hypha still different, different genetic relationship, thus showed the rich diversity.(2) Using the strainA137,A131,A172-2 with good growth situation of hypha respectively from Jiagedaqi District Forestry Bureau Dajin forestry station, Greater Xing an Academy of Agriculture and Forestry Sciences, longer distant relationship of Diqing, Yunnan and control group Yante5 to conduct a fruiting test, screened out the excellent strain with high yield and shorter growth duration is A137. Compared with the control group on yield up 7.9%, shorten the growth period of 3-5 days. To A137, the results of regional trial show that the bacterium strain is relatively shorter growth period, yield up to 78.7kgfresh mushroom each of 100 kgdry material, and have good stability. Good commodity traits, fruiting bodies ventral smooth, pleat in back.(3) The A137 as a tested strain, some or all of the alternatives to traditional sawdust as edible fungus culture medium, four substrates formula is designed, the traditional sawdust as a contrast, Each alternative formulation experiment repeated three times, the amount of repetition of 100 bags at a time. According to the yield from different formulations, trade properties, raw material costs, considering the various factors to select the amount of wood reed replace 38% of the formulation, production up to fresh mushroom 78.7kg each of 100 kgdry material, and fruiting bodies ventral smooth, pleats in back, daisy compact, good commodity characters,per million bags cost 1045 yuan less than the control group. Tailored to promote the use of reed areas.(4) In formula of different replacement level of reed, the change trend of six main extracellular enzyme activity both for rising first then reducing, but the peak period of the different extracellular enzyme activity is different, even some have two peaks, and the degradation rate of lignin, cellulose and hemicellulose with the activity change of lignin enzymes, cellulase and hemicellulase was a positive correlation.In the period of the high enzyme activity, the degradation rate of lignocellulose corresponding increased.. When the reed replacement amount reached 38%, the volume-weight of the matrix is relatively small, total porosity is relatively high, good for growth of mycelium growth, the decomposition rate of ligninis the highest. Clear the nutrition using rules of the A137 in the new matrix, play a guiding role for this strain and integrated cultivation technologies of the new matrix.