Study On Root Colonization Under Water Stress AMF And Rhizobium Of Amorpha Fruticosa

Amorpha fruticosa is perennial tufted deciduous undershrub, has the characteristics of strong adaptability, rapid growth, soil, etc., the existence of the strong ability of nitrogen and Rhizobium and arbuscular mycorrhizal fungi. Arbuscular Mycorrhizal fungi (AMF) is a kind of special symbiotic fungi,it can form a mutually beneficial symbiotic association with most plants in the natural world,can improve the nutritional status, Amorpha fruticosa promote growth and increase the resistance and disease resistance.Researchers conducted the study of rhizobia or arbuscular mycorrhizal fungi on Amorpha fruticosa and double inoculation inoculation,it proved that double inoculation with single inoculation on the growth promoting effect of Amorpha fruticosa,but under different water stress, of Amorpha fruticosa dual inoculation of mycorrhizae, nodule microscopic observation and on plant growth difference also has not yet been reported.This experiment to study Fructus Sophorae students seedlings as experimental material, a total of five moisture gradient, each gradient has AMF and Rhizobium and uninoculated treatment,at different stages of Amorpha fruticosa mycorrhizal and rhizobial microscopic structure and dynamic infection were observed and the effect on the growth of Amorpha fruticosa plant research.The main results are as follows:(1) Dual inoculation of Amorpha fruticosa has typical arbuscular mycorrhizal structures and Amorpha fruticosa AMF mycorrhizal microstructure in various water treatment under different expression.Can be observed under the microscope, AMF and Rhizobium double inoculation of Amorpha fruticosa mycorrhizal both intercellular and intracellular hyphae, also in mycorrhizal hyphae formed arbuscules and vesicles vesicles and spores fruit; in the nodule that AMF hyphae and vesicle;Discovery of AMF hyphae and vesicles in the nodule; which in different water stress under AMF continue to form the entry point to infection of Amorpha fruticosa root, but hyphae of Arbuscular structures and vesicles will slow digestion;Under mild stress AMF infection of Amorpha fruticosa root prior to other water stress group.Mild stress in Amorpha fruticosa growth stage increased the vesicular abundance, in plants at later growth stage of water saturated treatment group and severe stress can increase the vesicular abundance.(2) Dual inoculation of mycorrhizal fruticosa in different periods of different soil moisture content on the need;The infection rate was increased and then decreased with the growth time of plants under the same water gradient;Dual inoculation of a.fruticosa in the medium-term growth of mycorrhizal infection status of the best.The growth of Amorpha fruticosa early, mild stress promoted AMF on root infection;In the growth of Amorpha fruticosa â…¢ period under well watered conditions bubble cyst abundance was the highest, the fourth period of water saturated conditions of mycorrhizal infection intensity is maximum.(3) microstructure and lateral root of dual inoculation of Amorpha fruticosa root nodule with homology, and under different water stress of Amorpha fruticosa Root Nodule Microstructure of different effects and formation period is different; The nitrogen fixation ability could be improved by adding water to the root nodule;Amorpha growth stage is an important period of nodule formation, water reducing inhibition of nodule growth.The mature nodule microstructure consists of cortical tissue cells, meristem cells, sheath cells, vascular bundle system and central infected cell region;Amorpha fruticosa rhizobia isolated from cortical tissue cells, meristem cells and the center of the infected area formed first, and under severe water stress inhibited the development;Drought stress inhibited the development of sheath cells;With the increase of drought stress, the sheath cells inhibited their growth under moderate stress;The vascular system was not affected by water stress.The central tissue and infected cells were higher than the normal water treatment group under water saturation treatment;The number of nodule bacteria increased gradually with the growth period.(4) AMF and Rhizobium can alleviate or weaken the effect of drought on the growth of Amorpha fruticosa plants.The results showed that the maximum values of plant height, total biomass, stem diameter and leaf number were all in the normal water treatment group, while the minimum value was in the severe drought treatment group,that Amorpha fruticosa plants grew well in normal watering, excessive drought may inhibit the growth of plants,and by comparison, it is found that dual inoculation of Amorpha fruticosa measures compared with no inoculation of Amorpha fruticosa are improved and mycorrhizal dependency of is increased with the increase of water stress.