Anlysis Of Soil Microbial Diversity In The Western Region Of Inner Mongolia

Microbial diversity research is of great significance for exploitation and utilization of microbial resource and environmental protection. In this study, diversity and community structure of soil bacteria, fungi, and actinomycetes in western regions of Inner Mongolia were studied by PCR-DGGE (PCR-Denaturing Gradinent Gel Electrophoresis) technology.The results showed that in view of the characteristics of the high content of sand grains for the soil samples in the west of Inner Mongolia, the high quality soil total DNA could be obtained by the DNA extraction method which combined the physical, chemical and enzymatic methods.soil microbial resource in the western region of Inner Mongolia was very rich. The number of dominant microorganism in this region was relatively large, of which the bacterial richness was the highest. The dominant bacteria included Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Acidobacteria, Gemmatimonadetes, and Nitrospira. The dominant actinomycetes included Saccharopolyspora, Thermomonospora, Streptomyces, Iamiaceae, Corynebacterium, Timonella, and Pseudonocardia. The dominant fungi included Ascomycota and Basidiomycota.Microbial diversity analysis showed that the bacterial diversity was the most abundant, followed by actinomycetes, fungi minimum. Bacterial richness and Shannon index of the soil sample BT11 (castanozems) were the highest, while those of the sample WH2 (gray desery soil) were the lowest. The Bacterial evenness index of sample BT1 (castanozems) was the highest, while that of sample WH12 (Aeolian sandy soil) was the lowest.Fungus richness and Shannon index of the soil sample BT4 (castanozems) were the highest, while those of the sample BM26 (Aeolian sandy soil) were the lowest. The Fungus evenness index of sample BT10 (castanozems) was the highest, while that of sample BT1 (castanozems) was the lowest. Actinomycetes richness and Shannon index of the soil sample BT11 (castanozems) were the highest, while those of the sample WH2 (gray desery soil) and WH11 (Aeolian sandy soil) were the lowest. The Actinomycetes evenness index of sample BT12 (Grey cinnamon) was the highest, while that of sample BT34 (Solonchaks) was the lowest.In addition, Microbial distribution of the same soil type will be affected by latitude and longitude, soil utilization method and so on.